Single-cell RNA sequencing reveals the heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction

被引:3
|
作者
Chun, Jeewan [1 ,2 ]
Moon, Ji-Hoi [1 ]
Kwack, Kyu Hwan [1 ]
Jang, Eun-Young [1 ,2 ]
Lee, Saebyeol [3 ]
Kim, Hak Kyun [3 ]
Lee, Jae-Hyung [1 ]
机构
[1] Kyung Hee Univ, Coll Dent, Dept Oral Microbiol, Seoul 02447, South Korea
[2] Kyung Hee Univ, Grad Sch, Dept Dent, Seoul 02447, South Korea
[3] Chung Ang Univ, Dept Life Sci, Seoul 06974, South Korea
基金
新加坡国家研究基金会;
关键词
Chondrogenesis; Heterogeneity; Single-cell RNA sequencing (scRNA-seq); Transcriptome; Adipose tissue-derived mesenchymal stem cells (AT-MSCs); DIFFERENTIATION;
D O I
10.5483/BMBRep.2023-0161
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study investigated how adipose tissue-derived mesenchymal stem cells (AT-MSCs) respond to chondrogenic induction using droplet-based single-cell RNA sequencing (scRNA-seq). We analyzed 37,219 high-quality transcripts from control cells and cells induced for 1 week (1W) and 2 weeks (2W). Four distinct cell clusters (0-3), undetectable by bulk analysis, exhibited varying proportions. Cluster 1 dominated in control and 1W cells, whereas clusters (3, 2, and 0) exclusively dominated in control, 1W, and 2W cells, respectively. Furthermore, heterogeneous chondrogenic markers expression within clusters emerged. Gene ontology (GO) enrichment analysis of differentially expressed genes unveiled cluster-specific variations in key biological processes (BP): (1) Cluster 1 exhibited up-regulation of GO-BP terms related to ribosome biogenesis and translational control, crucial for maintaining stem cell properties and homeostasis; (2) Additionally, cluster 1 showed up-regulation of GO-BP terms associated with mitochondrial oxidative metabolism; (3) Cluster 3 displayed up-regulation of GO-BP terms related to cell proliferation; (4) Clusters 0 and 2 demonstrated similar up-regulation of GO-BP terms linked to collagen fibril organization and supramolecular fiber organization. However, only cluster 0 showed a significant decrease in GO-BP terms related to ribosome production, implying a potential correlation between ribosome regulation and the differentiation stages of AT-MSCs. Overall, our findings highlight heterogeneous cell clusters with varying balances between proliferation and differentiation before,
引用
收藏
页码:232 / 237
页数:6
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