Recent progress of methods for cuproptosis detection

被引:0
|
作者
Zhang, Ligang [1 ,2 ]
Deng, Ruiting [3 ]
Guo, Raoqing [4 ]
Jiang, Yawen [1 ]
Guan, Yichen [1 ]
Chen, Caiyue [1 ]
Zhao, Wudi [1 ]
Huang, Guobin [1 ]
Liu, Lian [1 ]
Du, Hongli [2 ]
Tang, Dongsheng [1 ]
机构
[1] Foshan Univ, Gene Editing Technol Ctr Guangdong Prov, Sch Med, Foshan, Peoples R China
[2] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou, Peoples R China
[3] Beijing Mercer United Int Educ Consulting Co Ltd, Guangzhou, Peoples R China
[4] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, State Key Lab Resp Dis, Guangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
cuproptosis; detection methods; cell death; Cu content; morphology; molecular biology; biochemical pathways; IRON-SULFUR CLUSTER; LIPOIC ACID; CELL-DEATH; COPPER; DISEASE; FERROPTOSIS; PROTEIN; METABOLISM; MATURATION; MUTATIONS;
D O I
10.3389/fmolb.2024.1460987
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Varying from other identified cell death pathways, cuproptosis is a new type of regulated cell death characterized by excess Cu ions, abnormal aggregation of lipoylated proteins in TCA cycle, loss of Fe-S cluster proteins, upregulation of HSP70, leading to proteotoxic and oxidative stress. Cuproptosis is highly concerned by scientific community and as the field of cuproptosis further develops, remarkable progress has been made in the verification and mechanism of cuproptosis, and methods used to detect cuproptosis have been continuously improved. According to the characteristic changes of cuproptosis, techniques based on cell death verification, Cu content, morphology, molecular biology of protein levels of cuproptosis-related molecules and biochemical pathways of cuproptosis-related enzyme activity and metabolites of oxidative stress, lipoic acid, TCA cycle, Fe-S cluster proteins, oxidative phosphorylation, cell respiration intensity have been subject to cuproptosis verification and research. In order to further deepen the understanding of detecting cuproptosis, the principle and application of common cuproptosis detection methods are reviewed and categorized in cellular phenomena and molecular mechanism in terms of cell death, Cu content, morphology, molecular biology, biochemical pathways with a flow chart. All the indicating results have been displayed in response to the markers of cuproptosis, their advantages and limitations are summaried, and comparison of cuproptosis and ferroptosis detection is performed in this study. Our collection of methods for cuproptosis detection will provide a great basis for cuproptosis verification and research in the future.
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页数:15
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