Characterization of a KPC-84 harboring Klebsiella pneumoniae ST11 clinical isolate with ceftazidime-avibactam resistance

被引：0
|
作者：
Gong, Yanqiao [1 ,2 ]
Feng, Yu [1 ,3 ]
Zong, Zhiyong [1 ,3 ,4 ]
Lv, Xiaoju [1 ,4 ]
机构：
[1] Sichuan Univ, West China Hosp, Ctr Infect Dis, Chengdu, Peoples R China
[2] Hubei Minzu Univ, Minda Hosp, Dept Infect Control, Enshi, Peoples R China
[3] Sichuan Univ, West China Hosp, Ctr Pathogen Res, Chengdu, Peoples R China
[4] Div Infect Dis, State Key Lab Biotherapy, Chengdu, Peoples R China
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2024年 / 43卷 / 10期
基金：
中国国家自然科学基金;
关键词：
KPC; Ceftazidime-avibactam; Klebsiella pneumoniae;
D O I：
10.1007/s10096-024-04910-y
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A novel KPC variant, KPC-84, identified in a Klebsiella pneumoniae isolate from China, exhibits a threonine (T) to proline (P) amino acid substitution at Ambler position 243(T243P), altering from the KPC-2 sequence. Cloning and expression of bla(KPC-84) in Escherichia coli, with subsequent MIC assessments, revealed increased resistance to ceftazidime-avibactam and significantly reduced carbapenemase activity compared to KPC-2. Kinetic measurements showed that KPC-84 exhibited sligthly higher hydrolysis of ceftazidime and reduced affinity for avibactam compared to KPC-2. This study emphasizes the emerging diversity of KPC variants with ceftazidime-avibactam resistance, underscoring the complexity of addressing carbapenem-resistant Klebsiella pneumoniae infections.
引用
收藏
页码：2029 / 2035
页数：7
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