The genome of Vicia sativa ssp. amphicarpa provides insights into the role of terpenoids in antimicrobial resistance within subterranean fruits

被引：0

作者：

Li, Mingyu ^{[1
]}

Pu, Jun ^{[1
]}

Jia, Chenglin ^{[1
]}

Luo, Dong ^{[1
]}

Zhou, Qiang ^{[1
]}

Fang, Xiangling ^{[1
]}

Nie, Bin ^{[1
]}

Liu, Wenxian ^{[1
]}

Nan, Zhibiao ^{[1
]}

Searle, Iain Robert ^{[2
]}

Fang, Longfa ^{[1
]}

Liu, Zhipeng ^{[1
]}

机构：

[1] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Herbage Improvement & Grassland Agro, Lanzhou 730020, Peoples R China

[2] Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia

来源：

PLANT JOURNAL | 2024年

关键词：

Vicia sativa ssp. amphicarpa; amphicarpic plants; genome assembly; antimicrobial activity in subterranean fruits; REPRODUCTIVE-BEHAVIOR; BIOSYNTHESIS; PLANTS; ALIGNMENT; QUANTIFICATION; METABOLISM; EVOLUTION; SEQUENCE; PASTURE; TOOLKIT;

D O I：

10.1111/tpj.16939

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Vicia sativa ssp. amphicarpa is a unique forage crop capable of simultaneously producing fruits above and below ground, representing a typical amphicarpic plant. In this study, we sequenced and assembled seven pseudo-chromosomes of the genome of V. sativa ssp. amphicarpa (n = 7) yielding a genome size of 1.59 Gb, with a total annotation of 48 932 protein-coding genes. Long terminal repeat (LTR) elements constituted 62.28% of the genome, significantly contributing to the expansion of genome size. Phylogenetic analysis revealed that the divergence between V. sativa ssp. amphicarpa and V. sativa was around 0.88 million years ago (MYA). Comparative transcriptomic and metabolomic analysis of aerial and subterranean pod shells showed biosynthesis of terpenoids in the subterranean pod shells indicating a correlation between the antimicrobial activity of subterranean pod shells and the biosynthesis of terpenoids. Furthermore, functional validation indicates that overexpression of VsTPS5 and VsTPS16 enhances terpenoid biosynthesis for antibacterial activity. Metabolomic analysis suggests the involvement of terpenoids in the antimicrobial properties of subterranean pod shells. Deciphering the genome of V. sativa ssp. amphicarpa elucidated the molecular mechanisms behind the antimicrobial properties of subterranean fruits in amphicarpic plants, providing valuable insights for the study of amphicarpic plant biology.

引用

页码：2654 / 2671

页数：18