Antiproliferative metabolites against glioma cells from the marine-associated actinomycete Streptomyces sp. ZZ735

Metabolites produced by the genus Streptomyces are the most important resource for discovering bioactive compounds. In this study, chemical investigation on the metabolites produced by the marine-derived Streptomyces sp. ZZ735 in rice solid medium led to the isolation of eighteen compounds (1-18). 1 - 18 ). Chemical structures of the isolated compounds were determined based on their HRESIMS data and the extensive NMR spectral analyses. Streptonaphthothiazines A (1), 1 ), B (2), 2 ), 2-(2-hydroxy-2-methylpropanoylamino)-benzoic acid (7), 7 ), and streptomycinoic acids A (17), 17 ), B (18) 18 ) are characterized as five previously undescribed compounds. The structural backbones of streptonaphthothiazines A (1), 1 ), B (2) 2 ) and streptomycinoic acids A (17), 17 ), B (18) 18 ) are found from a natural resource for the first time. It is also the first report of 2-(2-methylpropanoylamino)-benzoic acid (3), 3 ), 2-(2methylpropanoylamino)-benzamide (4), 4 ), methyl 2-(3-hydroxypropanoylamino)-benzoate (5), 5 ), 2-propionylaminobenzamide (6), 6 ), and (2E)-3-(3-hydroxy-4,5-dimethoxyphenyl)-2-propenoic E )-3-(3-hydroxy-4,5-dimethoxyphenyl)-2-propenoic acid (15) 15 ) as natural products. Streptonaphthothiazines A (1), 1 ), B (2) 2 ) and streptomycinoic acids A (17), 17 ), B (18) 18 ) have antiproliferative activity against human glioma U87MG or U251 cells with IC50 50 values ranging from 31.8 to 37.9 mu M.