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Performance evaluation of a newly developed 2019-nCoV nucleic acid detection kit based on Ion Proton sequencing platform and its comparison with the MGI Tech (DNBSEQ-G99) platform
被引：0
|作者：
He, Yuting
[1
]
Yang, Tingting
[1
]
Zhong, Guosheng
[2
]
Yu, Xuegao
[1
]
Zhao, Zhiwei
[1
]
Shi, Yaling
[3
]
Huang, Bin
[1
]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Clin Lab, 58 Zhongshan Rd 2, Guangzhou, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Clin Lab, Guangzhou, Peoples R China
[3] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Dept Clin Lab, Guangzhou, Peoples R China
关键词：
2019-nCoV nucleic acid detection kit;
Ion Proton;
MGI Tech (DNBSEQ-G99);
Next-generation sequencing;
Performance evaluation;
SARS-COV-2;
DIAGNOSIS;
COVID-19;
D O I：
10.1016/j.diagmicrobio.2024.116323
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Purpose: To evaluate the performance of a newly developed 2019-nCoV nucleic acid detection kit based on Ion Proton sequencing platform and make comparation with MGI Tech (DNBSEQ-G99) platform. Methods: References and clinical samples were used to evaluate the precision, agreement rate, limit of detection (LOD), anti-interference ability and analytical specificity. Twenty-seven clinical specimens were used to make comparison between two platforms. Results: The kit showed good intra-assay, inter-assay, inter-day precision between different operators and laboratories, fine agreement rate with references, a relatively low LOD of 1 x 10 3 copies/ml, anti-interference capability of 5 % whole blood and 1mg/ml mucin and no cross reaction with twenty-nine common clinical pathogens. Consistency of variant classification was observed between two platforms. The WGS from Ion Proton tended to have higher coverage and less missing data. Conclusions: The newly developed kit has shown satisfactory performances and excellent consistency with DNBSEQ-G99, making it a good alternative choice clinically.
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页数：8
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