Objective: The present study aimed to compare the static and dynamic sitting posture during computer work among symptomatic and asymptomatic office workers in actual work environment. Methods: Seventy female office workers were divided into two groups: asymptomatic (n = 35) and symptomatic (n = 35). Subsequent to this classification, adjustments to their respective workstations were implemented in accordance with Occupational Safety and Health Administration (OSHA) guidelines. The assessment of neck (CV) and shoulder (FS) angles were conducted during both a typical seated posture and at intervals of 20 min over a duration of 3 h of computer work. Result: The asymptomatic group had a range of age from 26 to 40 years, a BMI of 21.11 +/- 2.14, and a working experience ranged from 1 to 16 years. In contrast, the symptomatic group had an age range from 24 to 40 years, a BMI of 21.12 +/- 2.27, and a working experience ranged from 3 to 16 years. During static sitting posture, significant differences were observed in both CV (p = 0.01) and FS angles (p = 0.00) between the two groups. Additionally, during computer work sessions lasting for 3 h, a significant time effect (p = 0.00) was noted for the CV angle. Furthermore, the FS angle exhibited significant group (p = 0.00), time (p = 0.00), and interaction (p = 0.00) effects during work. Conclusion: This study underscores the development of neck flexion during prolong working in both groups. In addition, asymptomatic group experienced a progression more rounded shoulder during a 3-h working period. Prolonged periods of sitting and computer use appear to have adverse effects on neck and shoulder health, underlining the importance of implementing measures to mitigate these effects.