Cluster effect through the oligomerisation of bioactive disaccharide AMOR on pollen tube capacitation in Torenia fournieri

被引:0
|
作者
Mizukami, Akane G. [1 ,4 ]
Kusano, Shuhei [2 ]
Matsuura-Tokita, Kumi [1 ]
Hagihara, Shinya [2 ]
Higashiyama, Tetsuya [1 ,3 ]
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan
[2] RIKEN, Ctr Sustainable Resource Sci, Saitama, 3510198, Japan
[3] Nagoya Univ, Inst Transformat Biomol WPI ITbM, Nagoya 4648601, Japan
[4] Aichi Gakuin Univ, Div Liberal Arts & Sci, Nisshin, Aichi 4700195, Japan
来源
RSC CHEMICAL BIOLOGY | 2024年 / 5卷 / 08期
基金
日本科学技术振兴机构; 日本学术振兴会;
关键词
CLASSICAL ARABINOGALACTAN PROTEIN; GLYCOPROTEIN; GROWTH; GENE;
D O I
10.1039/d4cb00032c
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabinogalactan proteins (AGPs) are plant-specific glycoproteins involved in cellular mechanics and signal transduction. There has been major progress in understanding the structure, synthesis, and molecular functions of their carbohydrate chains; however, the mechanisms by which they function as signalling molecules remain unclear. Here, methyl-glucuronosyl arabinogalactan (AMOR; Me-GlcA-beta(1,6)-Gal), a disaccharide structure at the end of AGP carbohydrate chains, was oligomerised via chemical synthesis. The biological activity of AMOR oligomers was enhanced via clustering of the carbohydrate chains. Furthermore, AMOR oligomers yielded a pollen tube morphology (i.e., callose plug formation) similar to that when cultured with native AMOR, suggesting it may be functionally similar to native AMOR.
引用
收藏
页码:745 / 750
页数:6
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