Involvement of circRNA Regulators MBNL1 and QKI in the Progression of Esophageal Squamous Cell Carcinoma

被引:1
|
作者
Wang, Hai-Feng [1 ,2 ]
Zhou, Xiao-Feng [3 ]
Zhang, Qun-Mei [4 ]
Wu, Jie-Qing [2 ]
Hou, Jing-Han [3 ]
Xu, Xue-Lian [2 ]
Li, Xiu-Min [3 ]
Liu, Yu-Long [1 ,5 ,6 ]
机构
[1] Soochow Univ, Affiliated Hosp 2, Dept Oncol, 1055 Sanxiang Rd, Gusu Dist, Suzhou 215004, Jiangsu, Peoples R China
[2] Xinxiang Med Univ, Affiliated Hosp 1, Dept Oncol, Xinxiang, Henan, Peoples R China
[3] Xinxiang Med Univ, Affiliated Hosp 3, Henan Key Lab Tumor Mol Therapy Med, 83,East Sect Hualan Ave, Xinxiang 453003, Henan, Peoples R China
[4] Xinxiang Med Univ, Affiliated Hosp 1, Dept Blood Transfus, Xinxiang, Henan, Peoples R China
[5] Soochow Univ, Sch Radiat Med & Protect, State Key Lab Radiat Med & Protect, Suzhou, Jiangsu, Peoples R China
[6] Collaborat Innovat Ctr Radiol Med Jiangsu Higher E, Suzhou, Jiangsu, Peoples R China
关键词
ESCC; MBNL1; QKI; circRNA; tumor progression; BINDING PROTEIN QUAKING; DOWN-REGULATION; CANCER; SUPPRESSOR; OVEREXPRESSION; PROLIFERATION; ALIGNMENT;
D O I
10.1177/10732748241257142
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
ObjectivesTo investigate the role of circRNA regulators MBNL1 and QKI in the progression of esophageal squamous cell carcinoma.BackgroundMBNL1 and QKI are pivotal regulators of pre-mRNA alternative splicing, crucial for controlling circRNA production - an emerging biomarker and functional regulator of tumor progression. Despite their recognized roles, their involvement in ESCC progression remains unexplored.MethodsThe expression levels of MBNL1 and QKI were examined in 28 tissue pairs from ESCC and adjacent normal tissues using data from the GEO database. Additionally, a total of 151 ESCC tissue samples, from stage T1 to T4, consisting of 13, 43, 87, and 8 cases per stage, respectively, were utilized for immunohistochemical (IHC) analysis. RNA sequencing was utilized to examine the expression profiles of circRNAs, lncRNAs, and mRNAs across 3 normal tissues, 3 ESCC tissues, and 3 pairs of KYSE150 cells in both wildtype (WT) and those with MBNL1 or QKI knockouts. Transwell, colony formation, and subcutaneous tumorigenesis assays assessed the impact of MBNL1 or QKI knockout on ESCC cell migration, invasion, and proliferation.ResultsESCC onset significantly altered MBNL1 and QKI expression levels, influencing diverse RNA species. Elevated MBNL1 or QKI expression correlated with patient age or tumor invasion depth, respectively. MBNL1 or QKI knockout markedly enhanced cancer cell migration, invasion, proliferation, and tumor growth. Moreover, the absence of either MBNL1 or QKI modulated the expression profiles of multiple circRNAs, causing extensive downstream alterations in the expression of numerous lncRNAs and mRNAs. While the functions of circRNA and lncRNA among the top 20 differentially expressed genes remain unclear, mRNAs like SLCO4C1, TMPRSS15, and MAGEB2 have reported associations with tumor progression.ConclusionsThis study underscores the tumor-suppressive roles of MBNL1 and QKI in ESCC, proposing them as potential biomarkers and therapeutic targets for ESCC diagnosis and treatment.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Involvement of TSLC1 in progression of esophageal squamous cell carcinoma
    Ito, T
    Shimada, Y
    Hashimoto, Y
    Kaganoi, J
    Kan, T
    Watanabe, G
    Murakami, Y
    Imamura, M
    CANCER RESEARCH, 2003, 63 (19) : 6320 - 6326
  • [2] Involvement of chromosome region maintenance 1 (CRM1) in the formation and progression of esophageal squamous cell carcinoma
    Xiaojing Yang
    Lei Cheng
    Li Yao
    Hanru Ren
    Shu Zhang
    Xiao Min
    Xueyu Chen
    Jianguo Zhang
    Mei Li
    Medical Oncology, 2014, 31
  • [3] Involvement of chromosome region maintenance 1 (CRM1) in the formation and progression of esophageal squamous cell carcinoma
    Yang, Xiaojing
    Cheng, Lei
    Yao, Li
    Ren, Hanru
    Zhang, Shu
    Min, Xiao
    Chen, Xueyu
    Zhang, Jianguo
    Li, Mei
    MEDICAL ONCOLOGY, 2014, 31 (09) : 1 - 13
  • [4] NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis
    Tang, Tian
    Zeng, Feng
    JOURNAL OF IMMUNOLOGY RESEARCH, 2021, 2021
  • [5] Roles of circRNA dysregulation in esophageal squamous cell carcinoma tumor microenvironment
    Li, Jingyi
    Song, Yuxia
    Cai, Huihong
    Zhou, Bo
    Ma, Jun
    FRONTIERS IN ONCOLOGY, 2023, 13
  • [6] Role of Brg1 in progression of esophageal squamous cell carcinoma
    Torkamandi, Shahram
    Moghbeli, Meysam
    Farshchian, Moein
    Rad, Abolfazl
    Abbaszadegan, Mohammad Reza
    IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2014, 17 (11) : 912 - 916
  • [7] Role of DIDO1 in Progression of Esophageal Squamous Cell Carcinoma
    Mohammad Mahdi Forghanifard
    Pegah Naeimi Khorasanizadeh
    Mohammad Reza Abbaszadegan
    Afsaneh Javdani Mallak
    Meysam Moghbeli
    Journal of Gastrointestinal Cancer, 2020, 51 : 83 - 87
  • [8] HMGB1 is involved in esophageal squamous cell carcinoma progression
    Matsubara, Daiki
    Konishi, Hirotaka
    Takao, Koji
    Fukunaga, Tomohiko
    Nanishi, Kenji
    Ogino, Shinpei
    Shoda, Katsutoshi
    Arita, Tomohiro
    Kosuga, Toshiyuki
    Komatsu, Shuhei
    Shiozaki, Atsushi
    Okamoto, Kazuma
    Otsuji, Eigo
    CANCER SCIENCE, 2018, 109 : 468 - 468
  • [9] Role of DIDO1 in Progression of Esophageal Squamous Cell Carcinoma
    Forghanifard, Mohammad Mahdi
    Naeimi Khorasanizadeh, Pegah
    Abbaszadegan, Mohammad Reza
    Javdani Mallak, Afsaneh
    Moghbeli, Meysam
    JOURNAL OF GASTROINTESTINAL CANCER, 2020, 51 (01) : 83 - 87
  • [10] GASTRIC INVOLVEMENT OF ESOPHAGEAL SQUAMOUS-CELL CARCINOMA
    KUWANO, H
    BABA, K
    IKEBE, M
    KITAMURA, K
    ADACHI, Y
    MORI, M
    SUGIMACHI, K
    BRITISH JOURNAL OF SURGERY, 1992, 79 (04) : 328 - 330