Hydrothermally synthesized biofunctional ceria nanoparticles using orange peel extract: optimization, characterization, and antibacterial and antioxidant properties

In this research, cerium oxide nanoparticles were synthesized using orange peel extract via a hydrothermal method. An equal ratio of orange peel extract to cerium nitrate salt led to the formation of cerium hydroxide carbonate, whereas a 1 : 10 ratio formed cerium oxide. The hydrothermal treatment was conducted for durations of 5 and 25 hours. Scanning electron microscopy (SEM) images revealed that the hydrothermal samples treated for 5 hours exhibited significant agglomeration in both extract to salt ratios after heat treatment. X-ray diffraction patterns confirmed that all samples were converted into cerium oxide after heating at 500 degrees C for 3 hours. Based on XRD and SEM results, three cerium oxide samples, including those synthesized through the 25 hours hydrothermal process with a 1 : 10 ratio and the 25 hours hydrothermal process with both ratios and subsequent heat treatment, were selected for further investigation. Fourier transform infrared (FT-IR) analysis revealed more adsorption of the functional groups of orange peel extract on the surface of the as-synthesized sample. Moreover, the heat-treated sample with a 1 : 10 ratio, initially cerium oxide, displayed a higher amount of surface functional groups than the one with a 1 : 1 ratio which was initially cerium hydroxide carbonate. The antibacterial activities of the samples were determined using the colony count method. Activities of all samples against Gram-negative bacteria are in the range of 91.5-93.2% with a negligible difference, whereas the as-synthesized sample exhibited a superior activity of 96.6 +/- 1.8% against Gram-positive bacteria compared to the other two heat-treated samples. The 87.3% antioxidant activity of the as-synthesized sample significantly surpassed that of the other two samples, as evaluated by the DPPH radical scavenging method. The compounds of orange peel extract attach to the surface of the synthesized nanoparticles, enhancing the antibacterial and antioxidant activities of the nanoparticles. However, these activities decrease with heat treatment.