Role of long non-coding RNA TCONS_02443383 in regulating cell adhesion and peroxisome proliferator-activated receptor (PPAR) signaling genes in atherosclerosis: A New Zealand white rabbit model study

被引：0

作者：

Yilihamu, Yilinuer ^{[1
]}

Xu, Rui ^{[1
]}

Jia, Wenxiao ^{[1
]}

Kukun, Hanjiaerbieke ^{[1
]}

Aihemaiti, Dilinuerkezi ^{[1
]}

Chang, Yifan ^{[1
]}

Ding, Shuang ^{[1
]}

Wang, Yunling ^{[1
]}

机构：

[1] Xinjiang Med Univ, Affiliated Hosp 1, Dept Radiol, 137 South Liyushan Rd, Urumqi 830054, Xinjiang, Peoples R China

来源：

GENE | 2024年 / 927卷

关键词：

Aortic atherosclerosis; High-throughput sequencing; New Zealand white rabbit; PROMOTES; ANNOTATION; MECHANISMS; MUSCLE;

D O I：

10.1016/j.gene.2024.148694

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Objective: In this study, we performed RNA sequencing (RNA-seq) on the abdominal aorta tissue of New Zealand rabbits and investigated the potential association of lncRNA TCONS_02443383 with the development of AS through bioinformatics analysis of the sequencing data. The obtained results were further validated using quantitative real-time polymerase chain reaction (qRT-PCR). Method: We induced an AS model in New Zealand rabbits by causing balloon injury to the abdominal aorta vascular wall and administering a high-fat diet. We then upregulated the expression level of the lncRNA TCONS_02443383 by injecting lentiviral plasmids through the ear vein. RNA sequencing (RNA-seq) was performed on the abdominal aorta tissues. We conducted Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway and Gene Ontology (GO) analyses. Result: The overexpression of the lncRNA TCONS_02443383 led to an upregulation of peroxisome proliferatoractivated receptor (PPAR) signaling pathways as well as genes related to cell adhesion. Conclusion: The overexpression of the lncRNA TCONS_02443383 can inhibit the occurrence and development of AS by upregulating peroxisome proliferator-activated receptor (PPAR) signaling pathways and genes related to cell adhesion.

引用

页数：12