Protocol for fast clonal family inference and analysis from large-scale B cell receptor repertoire sequencing data

被引：1

作者：

Wang, Kaixuan ^{[1
]}

Cai, Linru ^{[1
]}

Wang, Hao ^{[1
,2
]}

Shan, Shiwen ^{[1
]}

Hu, Xihao ^{[3
]}

Zhang, Jian ^{[1
]}

机构：

[1] Tianjin Univ, Acad Med Engn & Translat Med, Tianjin, Peoples R China

[2] Tianjin Univ, Georgia Tech Shenzhen Inst GTSI, Shenzhen, Guangdong, Peoples R China

[3] GV20 Therapeut, Cambridge, MA USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 02期

基金：

中国国家自然科学基金;

关键词：

MECHANISM; PACKAGE;

D O I：

10.1016/j.xpro.2024.102969

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The expeditious identification and comprehensive analysis of clonal families from extensive B cell receptor (BCR) repertoire sequencing data are imperative for elucidating the intricacies of B cell immune responses. Here, we introduce a computational pipeline designed to swiftly deduce clonal families from bulk BCR heavy -chain sequencing data, accompanied by a suite of functional modules tailored to streamline post -clustering analysis. The outlined methodology encompasses guidelines for software installation, meticulous data preparation, and the systematic inference and analysis of clonal families. For complete details on the use and execution of this protocol, please refer to Wang et al. 1

引用

页数：23

共 50 条

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