BrARR10 contributes to 6-BA-delayed leaf senescence in Chinese flowering cabbage by activating genes related to CTK, GA and ABA metabolism

被引：1

作者：

Wang, Ci-mei ^{[1
,2
,4
]}

Zeng, Ze-xiang ^{[1
]}

Liu, Zong-li ^{[1
]}

Zhu, Jian-hua ^{[2
]}

Su, Xin-guo ^{[3
]}

Huang, Ri-ming ^{[4
]}

Wei, Wei ^{[1
]}

Shan, Wei ^{[1
]}

Kuang, Jian-fei ^{[1
]}

Lu, Wang-jin ^{[1
]}

Chen, Jian-ye ^{[1
]}

Yang, Ying-ying ^{[1
,2
]}

机构：

[1] South China Agr Univ, Coll Hort, Engn Res Ctr Southern Hort Prod Preservat, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China

[2] Shaoguan Univ, Sch Food Sci & Engn, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan 512005, Peoples R China

[3] Guangdong AIB Polytech Coll, Guangzhou 510507, Peoples R China

[4] South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

来源：

POSTHARVEST BIOLOGY AND TECHNOLOGY | 2024年 / 216卷

基金：

中国国家自然科学基金;

关键词：

Brassica rapa; 6-benzylaminopurine; Type-B ARR; Leaf senescence regulation; Plant hormone signal transduction; CYTOKININ SIGNAL PERCEPTION; B RESPONSE; TRANSCRIPTION FACTORS; GENOME-WIDE; REGULATORS; ARR10; REPRESSES; BINDING;

D O I：

10.1016/j.postharvbio.2024.113084

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The synthetic cytokinin (CTK) 6-benzylaminopurine (6-BA), known to inhibit chlorophyll degradation, effectively retards leaf senescence in various horticultural crops. Although extensively implicated in root growth, shoot development, and abiotic stress response, the role of type-B ARRs in leaf senescence regulation remains relatively understudied. Hence, the involvement and mechanistic contribution of type-B ARR proteins in 6-BAinduced delay of postharvest leaf senescence in Chinese flowering cabbage merits closer investigation. In the present study, exogenous 6-BA treatment notably mitigated the decline in maximum quantum yield (Fv/Fm) and total chlorophyll content, as well as the expression of chlorophyll catabolic genes BrPPH1, BrNYC1 and senescence-associated gene BrSAG12 during Chinese flowering cabbage storage. Importantly, BrARR10, a type-B cytokinin response regulator, was induced by 6-BA treatment. Further, BrARR10 was characterized as a nuclearlocalized transcriptional activator. Moreover, DAP-seq analysis identified BrARR10's potential target genes, which predominantly function within the phytohormone signal transduction pathway. EMSA, ChIP-qPCR and DLR assays revealed that BrARR10 directly bound the promoters of CTK biosynthesis genes (BrIPT5, BrLOG3), GA biosynthesis genes (BrGA20ox1, BrGA20ox3), and ABA catabolism genes (BrCYP707A1, BrCYP707A3) in vitro and in vivo, simultaneously activating their expression. Additionally, leaves of transgenic Arabidopsis overexpressing BrARR10 displayed increased expression of these genes, resulting in a delayed senescence phenotype. Overall, the novel 6-BA-ARR model in this study contributes a new insight into the transcriptional regulatory mechanisms underlying 6-BA-mediated plant leaf senescence and furnishing a new theoretical foundation for understanding plant hormone interaction in postharvest preservation.

引用

页数：11