Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

被引：0
|
作者：
Park, Seungbin [1 ]
Lipton, Megan [1 ]
Sun, Yujiao J. [2 ]
Dadarlat, Maria [1 ]
机构：
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] UCL, Inst Ophthalmol, 11-43 Bath St, London EC1V 9EL, England
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 02期
关键词：
PYRAMIDAL TRACT CELLS; MICROSTIMULATION; EXCITATION; ACTIVATION; SENSATIONS; NEURONS; DRIVER; LINES;
D O I：
10.1016/j.xpro.2024.103027
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two -photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head -fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al. 1
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页数：20
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