Effects of in utero delta-9-tetrahydrocannabinol (THC) exposure on fetal and infant musculoskeletal development in a preclinical nonhuman primate model

被引:1
|
作者
Moellmer, Samantha A. [1 ]
Hagen, Olivia L. [2 ]
Farhang, Parsa A. [3 ]
Duke, Victoria R. [1 ]
Fallon, Meghan E. [4 ]
Hinds, Monica T. [1 ]
McCarty, Owen J. T. [1 ]
Lo, Jamie O. [2 ,5 ]
Nakayama, Karina H. [1 ]
机构
[1] Oregon Hlth & Sci Univ, Dept Biomed Engn, Portland, OR 97239 USA
[2] Oregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Div Reprod & Dev Sci, Beaverton, OR USA
[3] Johns Hopkins Univ, Dept Mol Microbiol & Immunol, Baltimore, MD USA
[4] Yale Sch Med, Yale Cardiovasc Res Ctr, Dept Internal Med, Sect Cardiovasc Med, New Haven, CT USA
[5] Oregon Hlth & Sci Univ, Dept Obstet & Gynecol, Div Maternal Fetal Med, Portland, OR USA
来源
PLOS ONE | 2024年 / 19卷 / 07期
关键词
SKELETAL-MUSCLE; CANNABINOID RECEPTORS; ENDOCANNABINOID SYSTEM; CB2; RECEPTOR; DELTA(9)-TETRAHYDROCANNABINOL; CANNABIDIOL; HYPERTROPHY; INHIBITION; CELLS;
D O I
10.1371/journal.pone.0306868
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The endocannabinoid system (ECS) plays a major role in the maintenance of bodily homeostasis and adaptive response to external insults. It has been shown to regulate crucial physiological processes and behaviors, spanning nervous functions, anxiety, cognition, and pain sensation. Due to this broad activity, the ECS has been explored as a potential therapeutic target in the treatment of select diseases. However, until there is a more comprehensive understanding of how ECS activation by exogenous and endogenous ligands manifests across disparate tissues and cells, discretion should be exercised. Previous work has investigated how endogenous cannabinoid signaling impacts skeletal muscle development and differentiation. However, the effects of activation of the ECS by delta-9-tetrahydrocannabinol (THC, the most psychoactive component of cannabis) on skeletal muscle development, particularly in utero, remain unclear. To address this research gap, we used a highly translational non-human primate model to examine the potential impact of chronic prenatal THC exposure on fetal and infant musculoskeletal development. RNA was isolated from the skeletal muscle and analyzed for differential gene expression using a Nanostring nCounter neuroinflammatory panel comprised of 770 genes. Histomorphological evaluation of muscle morphology and composition was also performed. Our findings suggest that while prenatal THC exposure had narrow overall effects on fetal and infant muscle development, the greatest impacts were observed within pathways related to inflammation and cytokine signaling, which suggest the potential for tissue damage and atrophy. This pilot study establishes feasibility to evaluate neuroinflammation due to prenatal THC exposure and provides rationale for follow-on studies that explore the longer-term implications and functional consequences encountered by offspring as they continue to mature.
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页数:15
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