Identifying and avoiding radiation damage in macromolecular crystallography

被引：0
|
作者：
Shelley, Kathryn L. [1 ]
Garman, Elspeth F. [1 ,2 ,3 ]
机构：
[1] Univ Oxford, Dept Biochem, Dorothy Crowfoot Hodgkin Bldg,South Parks Rd, Oxford OX1 3QU, England
[2] Univ Washington, Dept Biochem, Seattle, WA 98195 USA
[3] Univ Washington, Inst Prot Design, Seattle, WA 98195 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2024年 / 80卷
关键词：
radiation damage; specific damage; global damage; B-net; B-net-percentile; COPPER NITRITE REDUCTASE; ROOM-TEMPERATURE; ACTIVE-SITE; NUCLEOPROTEIN COMPLEXES; CRYSTAL-STRUCTURE; PROTEIN CRYSTALS; SYNCHROTRON; LASER; SPECTROSCOPY; ABSORPTION;
D O I：
10.1107/S2059798324003243
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Radiation damage remains one of the major impediments to accurate structure solution in macromolecular crystallography. The artefacts of radiation damage can manifest as structural changes that result in incorrect biological interpretations being drawn from a model, they can reduce the resolution to which data can be collected and they can even prevent structure solution entirely. In this article, we discuss how to identify and mitigate against the effects of radiation damage at each stage in the macromolecular crystal structure-solution pipeline.
引用
收藏
页码：314 / 327
页数：14
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