Oxygenated versus non-oxygenated flush out during deceased donor liver procurement: The first proof-of-concept study in humans

被引：0

作者：

Fernandes, Eduardo de Souza Martins ^{[1
,2
,3
,4
,5
]}

Correa, Raphael Rodrigues ^{[6
,7
]}

Furtado, Rodrigo Lopes Leite ^{[6
]}

Bruggenwirth, Isabel M. A. ^{[8
]}

Yang, Cindy ^{[8
]}

de Mello, Felipe Pedreira Tavares ^{[2
,3
,4
]}

Andrade, Ronaldo de Oliveira ^{[2
,3
,4
]}

Pimentel, Leandro Moreira Savattone ^{[2
,3
,4
]}

Girao, Camila Liberato ^{[2
,3
,4
]}

Cesar, Camilla ^{[2
,3
,4
]}

Siqueira, Munique Ana Pimentel ^{[2
,3
,4
]}

Braga, Eduardo Pinho ^{[6
]}

Carvalho, Angela Cristina Gouvea ^{[9
]}

Porte, Robert J. ^{[8
,10
]}

Bouskela, Eliete ^{[1
,11
,12
]}

机构：

[1] Rio Janeiro State Univ, Dept Physiol Sci, Lab Clin & Expt Res Vasc Biol Biovasc, Rio De Janeiro, Brazil

[2] DASA Sao Lucas Hosp, Dept Surg, Sect Hepatobiliary Surg & Liver Transplantat, Rio De Janeiro, Brazil

[3] Adventista Silvestre Hosp, Dept Surg, Sect Hepatobiliary Surg & Liver Transplantat, Rio De Janeiro, Brazil

[4] Sao Francisco Assis Hosp, Liver Transplant, Rio De Janeiro, Brazil

[5] Univ Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Dept Surg, Rio de Janeiro, Brazil

[6] DASA Sao Lucas Hosp, Dept Surg, Rio De Janeiro, Brazil

[7] Univ Fed Rio de Janeiro, Inst Biophys Carlos Chagas Filho, Rio De Janeiro, Brazil

[8] Univ Med Ctr Groningen, Sect Hepatobiliary Surg & Liver Transplantat, Dept Surg, Groningen, Netherlands

[9] DASA Sao Lucas Hosp, Dept Pathol, Rio De Janeiro, Brazil

[10] Erasmus Univ, Med Ctr, Dept Surg, Sect Hepatopancreato Biliary Surg & Liver Transpla, Rotterdam, Netherlands

[11] Univ Estado Rio De Janeiro, Hosp Univ Pedro Ernesto HUPE, Ctr Pesquisas Clin Multiusuario CePeM, Obes Unit, Rio De Janeiro, Brazil

[12] Univ Estado Rio De Janeiro, Fac Med Sci, Postgrad Program Clin & Expt Physiopathol Fiscline, Rio De Janeiro, Brazil

来源：

ARTIFICIAL ORGANS | 2024年

关键词：

liver procurement; liver transplantation; organ donation; oxygenated preservation solution; static cold storage; MACHINE PERFUSION; REPERFUSION INJURY; TRANSPLANTATION; DONATION;

D O I：

10.1111/aor.14815

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

BackgroundLiver transplantation is used for treating end-stage liver disease, fulminant hepatitis, and oncological malignancies and organ shortage is a major limiting factor worldwide. The use of grafts based on extended donor criteria have become internationally accepted. Oxygenated machine perfusion technologies are the most recent advances in organ transplantation; however, it is only applied after a period of cold ischemia. Due to its high cost, we aimed to use a novel device, OxyFlush (R), based on oxygenation of the preservation solution, applied during liver procurement targeting the maintenance of ATP during static cold storage (SCS).MethodsTwenty patients were randomly assigned to the OxyFlush or control group based on a 1:1 ratio. In the OxyFlush group, the perfusion solution was oxygenated with OxyFlush (R) device while the control group received a non-oxygenated solution. Liver and the common bile duct (CBD) biopsies were obtained at three different time points. The first was at the beginning of the procedure, the second during organ preparation, and the third after total liver reperfusion. Biopsies were analyzed, and adenosine triphosphate (ATP) levels and histological scores of the liver parenchyma and CBD were assessed. Postoperative laboratory tests were performed.ResultsOxyFlush (R) was able to maintain ATP levels during SCS and improved the damage caused by the lack of oxygen in the CBD. However, OxyFlush (R) did not affect laboratory test results and histological findings of the parenchyma.ConclusionWe present a novel low-cost device that is feasible and could represent a valuable tool in organ preservation during SCS. The OxyFlush (R) Study presents a new low-cost device that oxygenates the perfusate during organ procurement which allows a maintenance of ATP and an improvement in the histological injury score of bile ducts during static cold storage without compromising procurement time.image

引用

页码：1297 / 1307

页数：11

共 2 条

[1] Oxygenated versus non-oxygenated flush out and storage of donor livers: An experimental study
Bruggenwirth, Isabel M. A.
van der Plas, Willemijn S.
van Leeuwen, Otto B.
Thorne, Adam M.
Rayar, Michel
de Meijer, Vincent E.
Porte, Robert J.
[J]. ARTIFICIAL ORGANS, 2022, 46 (02) : 201 - 209
[2] OXYGENATED VERSUS NON-OXYGENATED FLUSH OUT AND STORAGE OF DCD PORCINE LIVERS
Bruggenwirth, Isabel
van der Plas, Willemijn
van Leeuwen, Otto B.
Thorne, Adam M.
Adelmeijer, Jelle
Rayar, Michel
de Meijer, Vincent E.
Porte, Robert J.
[J]. TRANSPLANT INTERNATIONAL, 2021, 34 : 146 - 147

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