A highly efficient organogenesis system based on 6-benzylaminopurine and indole-6-butyric acid in Suaeda glauca- a medicinal halophyte under varying photoperiods

被引：0

作者：

Mohammadi, Mohammad Aqa ^{[1
,2
]}

Xu, Mengjie ^{[1
]}

Wang, Yining ^{[1
]}

Zhang, Zihao ^{[1
]}

Wai, Myat Hnin ^{[1
]}

Rizwan, Hafiz Muhammad ^{[3
]}

An, Chang ^{[1
,2
,4
]}

Aslam, Mohammad ^{[1
]}

Qin, Yuan ^{[1
,2
]}

Cheng, Yan ^{[1
,2
]}

Zhang, Chunyin ^{[5
]}

Wang, Gang ^{[6
]}

机构：

[1] Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Coll Hort,Coll Life Sci,Coll Plant Protect,Coll Ag, Fuzhou 350002, Peoples R China

[2] Fujian Agr & Forestry Univ, Pingtan Inst Sci & Technol, Fuzhou 350002, Peoples R China

[3] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen Key Lab Food Nutr & Hlth, Shenzhen 518060, Peoples R China

[4] Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

[5] Yancheng Lvyuan Salt Soil Agr Technol Co Ltd, Yancheng 224051, Peoples R China

[6] Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224051, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2024年 / 216卷

基金：

中国国家自然科学基金;

关键词：

Suaeda glauca; Organogenesis; Callus; Plant growth regulators; Photoperiods; IN-VITRO REGENERATION; SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; SHOOT REGENERATION; CALLUS INDUCTION; LIGHT-INTENSITY; GROWTH; EXPLANT;

D O I：

10.1016/j.indcrop.2024.118672

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Suaeda glauca (Bunge) is a pivotal halophyte species, well-known for its rich nutrient content and its importance as a superior vegetable and oil crop, offering significant health benefits for both human and animal consumption. However, the absence of an efficient regeneration technique has severely hindered its genetic development. Consequently, there is an urgent need to establish an effective in vitro organogenesis initiation method for this valuable plant. In this study, our primary objective was to develop a comprehensive protocol for micropropagating S. glauca , primarily capitalizing on the synergistic effects of 6-benzylaminopurine (6-BAP) and indole-6-butyric acid (IBA). We explored the impact of different combinations of 6-benzylaminopurine (6-BAP), 2,4-dichloroacetic acid (2,4-D), and IBA on callus induction, organogenesis, and rooting using various explants (cotyledons, hypocotyls, and leaves) under two different photoperiod regimes during in vitro culture. Our results unveiled noteworthy variations in several variables related to organogenesis. The optimal combination of plant growth regulators (PGRs) for inducing embryonic callus from cotyledon (COT) was pinpointed at 4.44 mu M 6-BAP and 2.71 mu M 2,4-D. By employing 6-BAP concentrations ranging from 8.88 mu M to 13.33 mu M, alongside 0.49 mu M IBA, 3% sucrose, and 0.3% phytagel in full MS media under light conditions, we achieved a remarkable 100% regeneration rate through the direct organogenesis pathway. Our findings underscore the pivotal role of 6-BAP in triggering multiple shoots and attaining a 3.6% callus differentiation rate, while 0.98 mu M IBA resulted in a 65.28% root induction rate, facilitating the successful acclimatization of plants. Based on the synergistic effects of 6-BAP and IBA, we propose a robust protocol for S. glauca organogenesis, which holds great promise for streamlining micropropagation in S. glauca tissue culture. This standardized protocol has the potential to advance genetic improvement and promote the commercial cultivation of S. glauca as a valuable halophyte species.

引用

页数：11