The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

被引：1

作者：

Chen, Xiaoyi ^{[1
]}

Torres, Juan P. Moran ^{[1
]}

Vonk, Peter Jan ^{[1
]}

Damen, J. Mirjam A. ^{[2
,3
]}

Reiding, Karli R. ^{[2
,3
]}

Dijksterhuis, Jan ^{[4
]}

Lugones, Luis G. ^{[1
]}

Wosten, Han A. B. ^{[1
]}

机构：

[1] Univ Utrecht, Dept Biol, Microbiol, Padualaan 8, NL-3584 CH Utrecht, Netherlands

[2] Univ Utrecht, Bijvoet Ctr Biomol Res, Biomol Mass Spectrometry & Prote, Padualaan 8, NL-3584 CH Utrecht, Netherlands

[3] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands

[4] Westerdijk Fungal Biodivers Inst, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

来源：

FUNGAL GENETICS AND BIOLOGY | 2024年 / 172卷

关键词：

Fungus; Aspergillus; FlbA; Asexual development; Secretion; Cell wall stress; ACTIVATOR XLNR; CELL-WALL; D-XYLOSE; FUNCTIONAL-ANALYSIS; GENE-EXPRESSION; ENCODING GENES; SECRETION; COLONIES; STRESS; GROWTH;

D O I：

10.1016/j.fgb.2024.103894

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in A flbA . Here, seven of these genes ( abaA , aslA , aslB , azf1, htfA , nosA , and srbA ) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of A aslA and A aslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of A abaA, A aslA , A nosA, and A srbA , while biomass was higher in liquid shaken cultures of A aslA and A aslB . The A aslA and A htfA strains showed increased resistance to H 2 O 2 , while A aslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of A flbA of A. niger .

引用

页数：13