A stress sensor, IRE1a, is required for bacterial-exotoxin-induced interleukin-1b production in tissue-resident macrophages

被引：0

作者：

Sasaki, Izumi ^{[1
]}

Fukuda-Ohta, Yuri ^{[1
,2
]}

Nakai, Chihiro ^{[1
]}

Wakaki-Nishiyama, Naoko ^{[1
]}

Okamoto, Chizuyo ^{[1
]}

Okuzaki, Daisuke ^{[3
]}

Morita, Shuhei ^{[4
]}

Kaji, Shiori ^{[5
]}

Furuta, Yuki ^{[6
]}

Hemmi, Hiroaki ^{[1
,7
]}

Kato, Takashi ^{[1
]}

Yamamoto, Asumi ^{[1
]}

Tosuji, Emi ^{[8
]}

Saitoh, Shin-Ichiroh ^{[9
]}

Tanaka, Takashi ^{[10
]}

Hoshino, Katsuaki ^{[11
]}

Fukuda, Shinji ^{[12
,13
,14
,15
]}

Miyake, Kensuke ^{[16
]}

Kuroda, Etsushi ^{[17
]}

Ishii, Ken J. ^{[18
]}

Iwawaki, Takao ^{[19
]}

Furukawa, Koichi ^{[20
]}

Kaisho, Tsuneyasu ^{[1
]}

机构：

[1] Wakayama Med Univ, Inst Adv Med, Dept Immunol, Wakayama 6418509, Japan

[2] RIKEN Ctr Brain Sci, Lab Prot Conformat Dis, Wako, Saitama 3510198, Japan

[3] Osaka Univ, Genome Informat Res Ctr, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan

[4] Wakayama Med Univ, Dept Med 1, Wakayama 6418509, Japan

[5] Wakayama Med Univ, Dept Internal Med 2, Wakayama 6418509, Japan

[6] Wakayama Med Univ, Dept Thorac & Cardiovasc Surg, Wakayama 6418509, Japan

[7] Okayama Univ Sci, Fac Vet Med, Lab Immunol, Imabari, Ehime 7948555, Japan

[8] Wakayama Med Univ, Dept Dermatol, Wakayama 6418509, Japan

[9] Wakayama Med Univ, Inst Adv Med, Dept Intractable Disorders, Wakayama 6418509, Japan

[10] RIKEN Ctr Integrat Med Sci, Lab Dev Genet, Yokohama, Kanagawa 2300045, Japan

[11] Kagawa Univ, Fac Med, Dept Immunol, Miki, Kagawa 7610793, Japan

[12] Keio Univ, Inst Adv Biosci, Tsuruoka, Yamagata 9970052, Japan

[13] Kanagawa Inst Ind Sci & Technol, Gut Environm Design Grp, Kawasaki, Kanagawa 2100821, Japan

[14] Univ Tsukuba, Transborder Med Res Ctr, Tsukuba, Ibaraki 3058575, Japan

[15] Juntendo Univ, Lab Regenerat Microbiol, Grad Sch Med, Tokyo 1138421, Japan

[16] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Innate Immun, Tokyo 1088639, Japan

[17] Hyogo Med Univ, Sch Med, Dept Immunol, Nishinomiya, Hyogo 6638501, Japan

[18] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Vaccine Sci, Tokyo 1088639, Japan

[19] Kanazawa Med Univ, Med Res Inst, Dept Life Sci, Div Cell Med, Uchinada, Ishikawa 9200293, Japan

[20] Chubu Univ, Coll Life & Hlth Sci, Dept Biomed Sci, Kasugai, Aichi 4878501, Japan

来源：

CELL REPORTS | 2024年 / 43卷 / 04期

关键词：

CHOLERA-TOXIN; INFLAMMASOME ACTIVATION; NLRP3; INFLAMMASOME; CELL-DEATH; MEMBRANE; PROTEINS; MICE; CYTOSOL; RELEASE;

D O I：

10.1016/j.celrep.2024.113981

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cholera toxin (CT), a bacterial exotoxin composed of one A subunit (CTA) and five B subunits (CTB), functions as an immune adjuvant. CTB can induce production of interleukin-1b (IL -1b), a proinflammatory cytokine, in synergy with a lipopolysaccharide (LPS), from resident peritoneal macrophages (RPMs) through the pyrin and NLRP3 inflammasomes. However, how CTB or CT activates these inflammasomes in the macrophages has been unclear. Here, we clarify the roles of inositol-requiring enzyme 1 alpha (IRE1a), an endoplasmic reticulum (ER) stress sensor, in CT -induced IL -1b production in RPMs. In RPMs, CTB is incorporated into the ER and induces ER stress responses, depending on GM1, a cell membrane ganglioside. IRE1a-deficient RPMs show a significant impairment of CT- or CTB-induced IL -1b production, indicating that IRE1a is required for CT- or CTB-induced IL -1b production in RPMs. This study demonstrates the critical roles of IRE1a in activation of both NLRP3 and pyrin inflammasomes in tissue -resident macrophages.

引用

页数：21

共 18 条

[1] The Phosphate Exporter xpr1b Is Required for Differentiation of Tissue-Resident Macrophages
Meireles, Ana M.
Shiau, Celia E.
Guenther, Catherine A.
Sidik, Harwin
Kingsley, David M.
Talbot, William S.
CELL REPORTS, 2014, 8 (06): : 1659 - 1667
[2] The interplay of. GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity
Afrin, Taiaba
Costello, Caitlin N.
Monella, Amber N.
Korner, Camilla J.
Pajerowska-Mukhtar, Karolina M.
PLANT SIGNALING & BEHAVIOR, 2022, 17 (01)
[3] A LIVER X-RECEPTOR-HYPOXIA INDUCIBLE FACTOR 1α INTERPLAY POTENTIATES INTERLEUKIN-1B PRODUCTION IN HUMAN MACROPHAGES.
Menegaut, L.
Thomas, C.
Jalil, A.
Steinmetz, E.
David, M.
ATHEROSCLEROSIS, 2019, 287 : E19 - E19
[4] Rebamipide Suppresses Monosodium Urate Crystal-Induced Interleukin-1b Production through Regulation of Oxidative Stress and Caspase-1 in THP-1 Cells
Choe, Jung-Yoon
Her, Minyoung
Kim, Seong-Kyu
ARTHRITIS & RHEUMATOLOGY, 2015, 67
[5] Morphological and immunological evidence of a unique selective production and endoplasmic reticular accumulation of interleukin-1α in rat peritoneal macrophages induced by Pseudomonas aeruginosa exotoxin A
Huang, YT
Jeng, CR
Cheng, CH
Chueh, LL
Liu, JJ
Pang, VF
CELLULAR IMMUNOLOGY, 2003, 221 (02) : 143 - 156
[6] BREFELDIN-A INHIBITS INTERLEUKIN-1B, BUT NOT PHORBOL ESTER-INDUCED PROSTAGLANDIN PRODUCTION IN HUMAN AMNION-DERIVED WISH CELLS
EVERSON, WV
FASEB JOURNAL, 1995, 9 (03): : A111 - A111
[7] IL-2 is required for the generation of viral-specific CD4+ Th1 tissue-resident memory cells and B cells are essential for maintenance in the lung
Hondowicz, Brian D.
Kim, Karen S.
Ruterbusch, Mikel J.
Keitany, Gladys J.
Pepper, Marion
EUROPEAN JOURNAL OF IMMUNOLOGY, 2018, 48 (01) : 80 - 86
[8] Cholera toxin B induces interleukin-1β production from resident peritoneal macrophages through the pyrin inflammasome as well as the NLRP3 inflammasome
Orimo, Takashi
Sasaki, Izumi
Hemmi, Hiroaki
Ozasa, Toshiya
Fukuda-Ohta, Yuri
Ohta, Tomokazu
Morinaka, Mio
Kitauchi, Mariko
Yamaguchi, Takako
Sato, Yayoi
Tanaka, Takashi
Hoshino, Katsuaki
Katayama, Kei-ichi
Fukuda, Shinji
Miyake, Kensuke
Yamamoto, Masahiro
Satoh, Takashi
Furukawa, Koichi
Kuroda, Etsushi
Ishii, Ken J.
Takeda, Kiyoshi
Kaisho, Tsuneyasu
INTERNATIONAL IMMUNOLOGY, 2019, 31 (10) : 657 - 668
[9] Impaired Phagocytic Function in CX3CR1+ Tissue-Resident Skeletal Muscle Macrophages Prevents Muscle Recovery After Influenza A Virus-Induced Pneumonia in Aged Mice
Runyan, C. E.
Welch, L. C.
Lecuona, E.
Shigemura, M.
Aillon, R.
Williams, K.
Watanabe, S.
Joshi, N.
Abdala-Valencia, H.
Markov, N.
McQuattie-Pimentel, A. C.
Lu, Z.
Sichizya, L.
Budinger, G.
Sznajder, J. I.
Misharin, A.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2020, 201
[10] Signal transduction mechanisms of HIV-1 gp120-induced proinflarnmatory cytokine interleukin-1 beta (IL-1b) production by primary human macrophages
Cheung, Rick W.
Ravyn, Vipa
Ptasznik, Andrzej
Collman, Ronald G.
JOURNAL OF NEUROVIROLOGY, 2007, 13 : 16 - 16

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