In vitro assessment of ferroptosis of cells exposed to cigarette smoke aerosol using a self-designed on-chip evaluation system based on gas - liquid dual-dimensional exposure

被引：1

作者：

Li, Zezhi ^{[1
,2
,3
]}

Li, Xiang ^{[1
,2
]}

Liu, Kejian ^{[2
]}

Zhao, Junwei ^{[1
,2
]}

Shang, Pingping ^{[2
]}

Hua, Chenfeng ^{[2
]}

Guo, Junwei ^{[2
]}

Xie, Fuwei ^{[2
]}

Xie, Jianping ^{[1
,2
]}

机构：

[1] Beijing Life Sci Acad, Beijing 102209, Peoples R China

[2] CNTC, Key Lab Tobacco Chem, Zhengzhou Tobacco Res Inst, 2 Fengyang St, Zhengzhou 450001, Peoples R China

[3] Beijing Technol & Business Univ, Beijing 100048, Peoples R China

来源：

TALANTA | 2024年 / 277卷

关键词：

Bionic-lung chip; Aerosol exposure system; Multi-throughput; Ferroptosis;

D O I：

10.1016/j.talanta.2024.126352

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aerosol pollutants significantly cause health concerns. Herein, we established an original real-time aerosol exposure system that used a self-designed bionic-lung microfluidic chip. The chip features a 4 x 4 intersecting array within gas and liquid layers, creating 16 distinct microenvironments. A membrane situated between the layers offers attachment for cells and establishes a gas-liquid interface. This design provides a reliable screening capacity for investigating the biological effects of aerosol exposure in vitro by manipulating the gas and/or liquid conditions. Using this system, we validated that cigarette smoke (CS) aerosol triggered a concentration- and timedependent reduction in cell viability and intracellular glutathione levels, accompanied by an increase in intracellular reactive oxygen species and Fe2+. Furthermore, CS aerosol significantly downregulated the expression of GPX4, SLC7A11, and FTL mRNA while inducing a notable increase in that of ACSL4 mRNA. Additionally, CS aerosol markedly stimulated the release of proinflammatory cytokines. Crucially, the ferroptosis inhibitor deferoxamine mesylate reversed these biological indicators. These results demonstrate that our novel bionic-lung chip presents a suitably achievable approach to investigate the biological effects induced by aerosol exposure.

引用

页数：10