Formulation of O-carboxymethyl chitosan with magnesium phosphate cement promotes in vitro / in vivo angiogenesis and osteogenesis related to the TRPM7 channel in bone regeneration

被引：1

作者：

Gong, Changtian ^{[1
,2
]}

Yang, Jian ^{[2
]}

Zheng, Di ^{[2
]}

Zhou, Bin ^{[2
,3
]}

Zhang, Xiping ^{[2
]}

Wang, Xingyu ^{[2
]}

Huang, Xinghan ^{[2
]}

Ye, Qingsong ^{[1
,4
,5
,6
]}

Guo, Weichun ^{[2
]}

机构：

[1] Wuhan Univ, Renmin Hosp, Ctr Regenerat Med, Dept Stomatol, Wuhan 430060, Peoples R China

[2] Wuhan Univ, Renmin Hosp, Dept Orthoped, Wuhan 430060, Peoples R China

[3] Ezhou Cent Hosp, Dept Orthoped, Ezhou 436000, Hubei, Peoples R China

[4] Linhai Second Peoples Hosp, Linhai 317000, Zhejiang, Peoples R China

[5] Wuhan Univ, Renmin Hosp, Ctr Regenerat Med, Wuhan 430060, Peoples R China

[6] Wuhan Univ, Renmin Hosp, Dept Stomatol, Wuhan 430060, Peoples R China

来源：

COMPOSITES PART B-ENGINEERING | 2024年 / 280卷

关键词：

Bone regeneration; Periodontitis; Magnesium phosphate cement; O -Carboxymethyl chitosan; TRPM7; channel;

D O I：

10.1016/j.compositesb.2024.111451

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Despite the abundant evidence on the biodegradability of K-struvite (KMgPO 4 & sdot; 6H 2 O) that promotes bone regeneration, the cell-related surface bioactivity still stays low owing to the absence of organic components. O - Carboxymethyl chitosan ( O -CMC) is a glycosaminoglycan-mimetic with outstanding biocompatibility, biodegradability and solubility, which has been successfully used in tissue engineering and regenerative medicine (TERM). In this study, we incorporated O -CMC into K-struvite to prepare hybrid scaffolds (OMPCs) with increasing biphasic contents. We found that O -CMC improved the physicochemical properties, in vitro/vivo biocompatibility, and enzymatic biodegradability of K-struvite, resulting in enhanced in vitro/vivo angiogenesis and osteogenesis via the proper release of Mg 2 + . In addition, we found differential expression of transient receptor potential cation channel member 7 (TRPM7) within the endothelial and osteoblast lineages, triggering the phosphorylation of the PI3K/Akt and MEK/ERK signaling pathways, respectively. In summary, it can be concluded that the biphasic addition of O -CMC is a viable method for improving the surface bioactivity of Kstruvite to accelerate bone regeneration.

引用

页数：16