Heat shock protein (HSP90) affects the replication of rotavirus

被引：0
|
作者：
Hu, Siman [1 ]
Tao, Xiao-Li [1 ]
Zhao, Wei [1 ]
Luo, Like [1 ]
Li, Runli [1 ]
Ma, Zhuping [1 ]
Sun, Lvyin [1 ]
Li, Yong-Gang [1 ]
机构：
[1] Jinzhou Med Univ, Dept Pathogen Microbiol, Jinzhou 121000, Liaoning, Peoples R China
来源：
FUTURE VIROLOGY | 2024年 / 19卷 / 6-7期
关键词：
17-AAG; HSP90; NSP2; proteasome pathway; RV; NONSTRUCTURAL PROTEIN; ACTIVE PARTICIPATION; CHAPERONE HSP90; NSP2; RNA; COMPLEX; DEGRADATION; INHIBITION; MULTIMERS; APOPTOSIS;
D O I：
10.1080/17460794.2024.2362016
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To explore the effects of HSP90 on rotavirus (RV) replication. Materials & methods: The interaction between NSP2 and HSP90 was analyzed by immunoprecipitation assay, and co-localization confirmed by an immunofluorescence assay. The viral titer was measured by immunofluorescence assay and viral protein expression investigated by western blotting. Results: The interaction between NSP2 and HSP90 was confirmed. Knockdown of HSP90 using siRNA resulted in a decrease in NSP2 expression and virus titer. HSP90 inhibitor 17-AAG significantly decreased NSP2 and NSP5 expression, as well as the virus titer, while proteasome inhibitor MG132 restored the protein amount and the viral titer. Conclusion: HSP90 is involved in rotavirus replication, and inhibition of HSP90 promotes the degradation of NSP2 through the proteasome pathway.
引用
收藏
页码：263 / 272
页数：10
相关论文
共 50 条
  • [1] Heat Shock Protein 90 (Hsp90) Preface
    Picard, Didier
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2012, 1823 (03): : 605 - 606
  • [2] Interaction of cepharanthine with immobilized heat shock protein 90α (Hsp90α) and screening of Hsp90α inhibitors
    Haginaka, Jun
    Kitabatake, Tomoko
    Hirose, Iyo
    Matsunaga, Hisami
    Moaddel, Ruin
    ANALYTICAL BIOCHEMISTRY, 2013, 434 (01) : 202 - 206
  • [3] Heat Shock Protein 90 (Hsp90) in Fungal Growth and Pathogenesis
    Lamoth F.
    Juvvadi P.R.
    Steinbach W.J.
    Current Fungal Infection Reports, 2014, 8 (4) : 296 - 301
  • [4] Role of Heat Shock Protein 90 (Hsp90) in Insulin Secretion
    Tamarina, Natalia A.
    Philipson, Louis H.
    DIABETES, 2013, 62 : A575 - A576
  • [5] Redefining the Phenotype of Heat Shock Protein 90 (Hsp90) Inhibitors
    Wang, Yao
    Koay, Yen Chin
    McAlpine, Shelli R.
    CHEMISTRY-A EUROPEAN JOURNAL, 2017, 23 (09) : 2010 - 2013
  • [6] Circulating heat shock protein 90 (Hsp90) and autoantibodies to Hsp90 are increased in patients with atopic dermatitis
    Sitko, Krzysztof
    Bednarek, Marta
    Mantej, Jagoda
    Trzeciak, Magdalena
    Tukaj, Stefan
    CELL STRESS & CHAPERONES, 2021, 26 (06): : 1001 - 1007
  • [7] Circulating heat shock protein 90 (Hsp90) and autoantibodies to Hsp90 are increased in patients with atopic dermatitis
    Krzysztof Sitko
    Marta Bednarek
    Jagoda Mantej
    Magdalena Trzeciak
    Stefan Tukaj
    Cell Stress and Chaperones, 2021, 26 : 1001 - 1007
  • [8] Heat Shock Protein 90 (Hsp90) Expression and Breast Cancer
    Zagouri, Flora
    Bournakis, Evangelos
    Koutsoukos, Konstantinos
    Papadimitriou, Christos A.
    PHARMACEUTICALS, 2012, 5 (09): : 1008 - 1020
  • [9] RET Is a Heat Shock Protein 90 (HSP90) Client Protein and Is Knocked Down upon HSP90 Pharmacological Block
    Alfano, Luigi
    Guida, Teresa
    Provitera, Livia
    Vecchio, Giancarlo
    Billaud, Marc
    Santoro, Massimo
    Carlomagno, Francesca
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2010, 95 (07): : 3552 - 3557
  • [10] Reactive cysteines of the 90-kDa heat shock protein, Hsp90
    Nardai, G
    Sass, B
    Eber, J
    Orosz, G
    Csermely, P
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 384 (01) : 59 - 67
12345