Synchronous analysis of acetaminophen, codeine, and caffeine in human fluids employing graphite screen-printed electrodes

A facile electrochemical approach is proposed for the synchronous determination of acetaminophen (ACP), codeine (COD) and caffeine (CAF) utilizing unmodified screen-printed electrodes (SPEs). The determination of ACP, COD and CAF has been explored across different supporting electrolytes including sulfuric acid (H2SO4), hydrochloric acid (HCl), phosphoric acid (H3PO4) and Briton Robinson (B.R) buffer solutions. It was found that a 0.05 mol L-1 sulfuric acid solution is an optimal supporting electrolyte utilized for voltammetric analysis of ACP, COD, and CAF with improved sensitivity, stability, and reproducibility. The electro-analytical sensing of ACP, COD and CAF was investigated using SPEs within linear concentration ranges of 3.0-35.0 mu mol L-1, 10-160 mu mol L-1 and 10-160 mu mol L-1 and revealed competitively low limits of detection (3S/N) of 0.9, 4.8 and 6.3 mu mol L-1 for ACP, COD and CAF, respectively. The results indicated the possibility of such a simple and quick electroanalytical protocol for online monitoring of pharmaceutical formulations comprising ACP, COD, and CAF drugs in human fluids with satisfactory recovery. Synchronous analysis of acetaminophen, codeine, and caffeine by screen-printed electrodes (SPEs) was explored. The SPEs showed high sensitivity as non-enzyme sensor, thereby obviating the necessity for laborious sample pre-treatment steps.