Supplementation of Mangiferin to a High-Starch Diet Alleviates Hepatic Injury and Lipid Accumulation Potentially through Modulating Cholesterol Metabolism in Channel Catfish (Ictalurus punctatus)

被引:2
|
作者
Zheng, Yutong [1 ,2 ]
Lu, Qisheng [2 ,3 ]
Cao, Jingyue [2 ,3 ]
Liu, Yulong [2 ,3 ]
Liu, Haokun [2 ]
Jin, Junyan [2 ]
Zhang, Zhimin [2 ]
Yang, Yunxia [2 ]
Zhu, Xiaoming [2 ]
Han, Dong [2 ,3 ,4 ]
Xie, Shouqi [2 ,3 ,5 ]
机构
[1] Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
[2] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[3] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China
[4] Hubei Hongshan Lab, Wuhan 430070, Peoples R China
[5] Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
mangiferin; starch; cholesterol metabolism; sqle; channel catfish; FATTY LIVER; SQUALENE EPOXIDASE; GROWTH-PERFORMANCE; PATHOGENESIS; INSIGHTS;
D O I
10.3390/antiox13060722
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Starch is a common source of carbohydrates in aqua feed. High-starch diet can cause hepatic injury and lipid accumulation in fish. Mangiferin (MGF) can regulate lipid metabolism and protect the liver, but there is limited research on its effects in fish. In the present study, we investigated whether MGF could ameliorate high-starch-induced hepatic damage and lipid accumulation in channel catfish. The channel catfish (Ictalurus punctatus) were fed one of four experimental diets for eight weeks: a control diet (NCD), a high-starch diet (HCD), an HCD supplemented with 100 mg/kg MGF (100 MGF), and an HCD supplemented with 500 mg/kg MGF (500 MGF). The results demonstrated that the weight gain rate (WGR) (p = 0.031), specific growth rate (SGR) (p = 0.039), and feed conversion efficiency (FCE) (p = 0.040) of the 500 MGF group were significantly higher than those of the NCD group. MGF supplementation alleviated liver damage and improved antioxidant capacity (T-AOC) compared to those of the HCD group (p = 0.000). In addition, dietary MGF significantly reduced plasma glucose (GLU) (p = 0.000), triglyceride (TG) (p= 0.001), and low-density lipoprotein cholesterol (LDL) (p = 0.000) levels. It is noteworthy that MGF significantly reduced the plasma total cholesterol (TC) levels (p = 0.000) and liver TC levels (p = 0.005) of channel catfish. Dietary MGF improves cholesterol homeostasis by decreasing the expression of genes that are involved in cholesterol synthesis and transport (hmgcr, sqle, srebf2, sp1, and ldlr) and increasing the expression of genes that are involved in cholesterol catabolism (cyp7a1). Among them, the largest fold decrease in squalene epoxidase (sqle) expression levels was observed in the 100 MGF or 500 MGF groups compared with the HCD group, with a significant decrease of 3.64-fold or 2.20-fold (p = 0.008). And the 100 MGF or 500 MGF group had significantly decreased (by 1.67-fold or 1.94-fold) Sqle protein levels compared to those of the HCD group (p = 0.000). In primary channel catfish hepatocytes, MGF significantly down-regulated the expression of sqle (p = 0.030) and reduced cholesterol levels (p = 0.000). In NCTC 1469 cells, MGF significantly down-regulated the expression of sqle (p = 0.000) and reduced cholesterol levels (p = 0.024). In conclusion, MGF effectively inhibits sqle expression and reduces cholesterol accumulation. The current study shows how MGF supplementation regulates the metabolism and accumulation of cholesterol in channel catfish, providing a theoretical basis for the use of MGF as a dietary supplement in aquaculture.
引用
收藏
页数:21
相关论文
共 5 条
  • [1] Effects of dietary curcumin supplementation on the liver health of juvenile channel catfish ( Ictalurus punctatus) fed high-starch diet
    Lu, Qisheng
    Zheng, Yutong
    Wang, Yu
    Liu, Yulong
    Cao, Jingyue
    Liu, Haokun
    Jin, Junyan
    Zhang, Zhimin
    Yang, Yunxia
    Zhu, Xiaoming
    Xie, Shouqi
    Han, Dong
    AQUACULTURE REPORTS, 2025, 40
  • [2] Mangiferin reduces high-starch diet-induced lipid accumulation and liver damage by modulating triglyceride metabolism in largemouth bass (Micropterus salmoides)
    Zheng, Yutong
    Lu, Qisheng
    Cao, Jingyue
    Han, Guoli
    Liu, Yulong
    Liu, Haokun
    Jin, Junyan
    Zhang, Zhimin
    Yang, Yunxia
    Zhu, Xiaoming
    Han, Dong
    Xie, Shouqi
    AQUACULTURE REPORTS, 2024, 37
  • [3] Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet
    Desouky, Hesham Eed
    Jiang, Guang-zhen
    Zhang, Ding-dong
    Abasubong, Kenneth Prudence
    Yuan, Xiangyang
    Li, Xiang-fei
    Liu, Wen-bin
    FISH PHYSIOLOGY AND BIOCHEMISTRY, 2020, 46 (02) : 653 - 663
  • [4] Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet
    Hesham Eed Desouky
    Guang-zhen Jiang
    Ding-dong Zhang
    Kenneth Prudence Abasubong
    Xiangyang Yuan
    Xiang-fei Li
    Wen-bin Liu
    Fish Physiology and Biochemistry, 2020, 46 : 653 - 663
  • [5] Dietary betulinic acid alleviates high carbohydrate diet-induced hepatic de novo lipogenesis through AMPK signaling and improves liver health in channel catfish ( Ictalurus punctatus )
    Liu, Yulong
    Lu, Qisheng
    Han, Guoli
    Zheng, Yutong
    Cao, Jingyue
    Xi, Longwei
    Liu, Haokun
    Jin, Junyan
    Zhang, Zhimin
    Yang, Yunxia
    Zhu, Xiaoming
    Han, Dong
    Xie, Shouqi
    AQUACULTURE REPORTS, 2024, 36