Glutamate Receptor-like (GLR) Family in Brassica napus: Genome-Wide Identification and Functional Analysis in Resistance to Sclerotinia sclerotiorum

被引:0
|
作者
Gulzar, Rana Muhammad Amir [1 ]
Ren, Chun-Xiu [1 ]
Fang, Xi [1 ]
Xu, You-Ping [2 ]
Saand, Mumtaz Ali [3 ]
Cai, Xin-Zhong [1 ,4 ]
机构
[1] Zhejiang Univ, Inst Biotechnol, Coll Agr & Biotechnol, Key Lab Biol & Ecol Control Crop Pathogens & Insec, Hangzhou 310058, Peoples R China
[2] Zhejiang Univ, Ctr Anal & Measurement, 866 Yu Hang Tang Rd, Hangzhou 310058, Zhejiang, Peoples R China
[3] Shah Abdul Latif Univ, Dept Bot, Khairpur 66020, Sindh, Pakistan
[4] Zhejiang Univ, Hainan Inst, Sanya 572025, Peoples R China
关键词
bioinformatics; Brassica napus; glutamate receptor; resistance; Sclerotinia sclerotiorum; ARABIDOPSIS; GENE; DIVERSITY; CALCIUM;
D O I
10.3390/ijms25115670
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plant glutamate receptor-like channels (GLRs) are homologs of animal ionotropic glutamate receptors. GLRs are critical in various plant biological functions, yet their genomic features and functions in disease resistance remain largely unknown in many crop species. Here, we report the results on a thorough genome-wide study of the GLR family in oilseed rape (Brassica napus) and their role in resistance to the fungal pathogen Sclerotinia sclerotiorum. A total of 61 GLRs were identified in oilseed rape. They comprised three groups, as in Arabidopsis thaliana. Detailed computational analyses, including prediction of domain and motifs, cellular localization, cis-acting elements, PTM sites, and amino acid ligands and their binding pockets in BnGLR proteins, unveiled a set of group-specific characteristics of the BnGLR family, which included chromosomal distribution, motif composition, intron number and size, and methylation sites. Functional dissection employing virus-induced gene silencing of BnGLRs in oilseed rape and Arabidopsis mutants of BnGLR homologs demonstrated that BnGLR35/AtGLR2.5 positively, while BnGLR12/AtGLR1.2 and BnGLR53/AtGLR3.2 negatively, regulated plant resistance to S. sclerotiorum, indicating that GLR genes were differentially involved in this resistance. Our findings reveal the complex involvement of GLRs in B. napus resistance to S. sclerotiorum and provide clues for further functional characterization of BnGLRs.
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页数:23
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