From waste to strength: Tailor-made enzyme activation design transformation of denatured soy meal into high-performance all-biomass adhesive

被引：3

作者：

Li, Xinyi ^{[1
,2
]}

Chen, Shiqing ^{[1
,2
]}

Shao, Jiawei ^{[1
,2
]}

Bai, Mingyang ^{[1
,2
]}

Zhang, Zhicheng ^{[1
,2
]}

Song, Pingan ^{[3
]}

Jiang, Shuaicheng ^{[1
,2
]}

Li, Jianzhang ^{[1
,2
]}

机构：

[1] Beijing Forestry Univ, State Key Lab Efficient Prod Forest Resources, MOE Key Lab Wood Mat Sci & Applicat, Beijing 100083, Peoples R China

[2] Beijing Forestry Univ, Beijing Key Lab Wood Sci & Engn, Beijing 100083, Peoples R China

[3] Univ Southern Queensland, Ctr Future Mat, Sch Agr & Environm Sci, Springfield 4300, Australia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 273卷

基金：

中国国家自然科学基金;

关键词：

Denatured soy meal adhesive; Double-enzyme co-activation method; Transglutaminase; Bonding strength; Molecular recombination;

D O I：

10.1016/j.ijbiomac.2024.133054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Given the severe protein denaturation and self -aggregation during the high -temperature desolubilization, denatured soy meal (DSM) is limited by its low reactivity, high viscosity, and poor water solubility. Preparing lowcost and high-performance adhesives with DSM as the key feedstock is still challenging. Herein, this study reveals a double -enzyme co -activation method targeting DSM with the glycosidic bonds in protein -carbohydrate complexes and partial amide bonds in protein, increasing the protein dispersion index from 10.2 % to 75.1 % improves the reactivity of DSM. The green crosslinker transglutaminase (TGase) constructs a robust adhesive isopeptide bond network with high water-resistant bonding strength comparable to chemical crosslinkers. The adhesive has demonstrated high dry/wet shear strength (2.56 and 0.93 MPa) for plywood. After molecular recombination by enzyme strategy, the adhesive had the proper viscosity, high reactivity, and strong water resistance. This research showcases a novel perspective on developing a DSM-based adhesive and blazes new avenues for changes in protein structural function and adhesive performance.

引用

页数：10