Exploring photobiological performance of ternary chemical combinations: Ultrasonic, spectroscopic investigations, photochemical reactivity, topology and antimicrobial evaluation of acetic acid trihydrate, 2-[(2-aminoacetyl) amino] acetic acid with methanamide

Amino acids are the essential, structural units of proteins, peptides and assured types of hormones and antibiotics partake in all the physiological processes of a living cell. Protein cannot occur without the correct amalgamation of amino acids. Utmost of the studies on amino acids and biomolecules have been agreed out in pure and mixed aqueous/non-aqueous solutions. The aim of the present experimental investigation was to explore the interionic interactions between amino acid with electrolyte ternary solutions. In order to understand the intermolecular interactions, FT-IR, UV-Visible, 1HNMR and biological analysis were carried out. The spectral analysis had revealed that ternary mixture formed a trimolecular associated entity through H-bonding and electrostatic effects. HOMO LUMO energies and energy gap were also recognized. The lowest energy gap is observed for HOMOLUMO energy transition of ternary mixture at 3.6712 eV. MEP and topology parameters were achieved on Acetic Acid Trihydrate with 2-[(2-aminoacetyl) amino] acetic acid. Also thermodynamic properties (internal pressure, free volume) with respect to temperature were premeditated. In vitro, studies of the ternary combinations of the title compound are done against the 5C1C, 2FMA, 5IHR, 5NC0, 5EB4 biological proteins (E.Coli, Aspergillus niger).The 5NCO protein with ligand is observed least binding energy of -5.16 kcal/mol. Both theoretical and experimental studies suggest that the title molecule has been found to reduce several risk factors.