Xin-Yi-Qing-Fei-Tang and its critical components reduce asthma symptoms by suppressing GM-CSF and COX-2 expression in RBL-2H3 cells

被引：2

作者：

Wang, Shulhn-Der ^{[1
]}

Chen, Po-Ting ^{[2
]}

Hsieh, Miao-Hsi ^{[3
]}

Wang, Jiu-Yao ^{[3
,4
]}

Chiang, Chung-Jen ^{[5
]}

Lin, Li-Jen ^{[6
]}

机构：

[1] China Med Univ, Coll Chinese Med, Sch Postbaccalaureate Chinese Med, Taichung 40402, Taiwan

[2] Southern Taiwan Univ Sci & Technol, Dept Biotechnol & Food Technol, Tainan 71005, Taiwan

[3] China Med Univ Hosp, Ctr Allergy Immunol & Microbiome AIM, Taichung, Taiwan

[4] China Med Univ, Childrens Hosp, Taichung, Taiwan

[5] China Med Univ, Dept Med Lab Sci & Biotechnol, 91 Hsueh Shih Rd, Taichung 40402, Taiwan

[6] China Med Univ, Coll Chinese Med, Sch Chinese Med, Taichung 40402, Taiwan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2024年 / 330卷

关键词：

Xin-Yi-Qing-Fei-Tang; Asthma; Dermatophagoides pteronyssinus; Mast cells; Timosaponin AIII; Genkwanin; MAST-CELLS; INFLAMMATION; GENKWANIN; BENEFITS; MEDICINE; CHILDREN; RISKS; DRUG;

D O I：

10.1016/j.jep.2024.118105

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: The traditional Chinese medicine (TCM) XYQFT is composed of 10 herbs. According to the NHIRD, XYQFT is one of the top ten most commonly used TCM prescriptions for asthma treatment. Aim of the study: The aim of this study was to explore whether XYQFT reduces asthma symptoms in a mouse model of chronic asthma and determine the immunomodulatory mechanism of mast cells. Materials and methods: BALB/c mice were intratracheally (it) stimulated with 40 mu L (2.5 mu g/mu L) of Dermatophagoides pteronyssinus (Der p) once a week for 6 consecutive weeks and orally administered XYQFT at 1 g/kg 30 min before Der p stimulation. Airway hypersensitivity, inflammatory cells in the BALF and total IgE in the blood were assessed in mice. In addition, RBL-2H3 cells (mast cells) were stimulated with DNP-IgE, after which different concentrations of XYQFT were added for 30 min to evaluate the effect of XYQFT on the gene expression and degranulation of DNP-stimulated RBL-2H3 cells. After the compounds in XYQFT were identified using LC- MS/MS, the PBD method was used to identify the chemical components that inhibited the expression of the GMCSF and COX-2 genes in mast cells. Results: The airway hypersensitivity assay demonstrated that XYQFT significantly alleviated Der p-induced airway hypersensitivity. Moreover, cell counting and typing of bronchoalveolar lavage fluid revealed a significant reduction in Der p-induced inflammatory cell infiltration with XYQFT treatment. ELISA examination further indicated a significant decrease in Der p-induced total IgE levels in serum following XYQFT administration. In addition, XYQFT inhibited the degranulation and expression of genes (IL-3, IL-4, ALOX-5, IL-13, GM-CSF, COX-2, TNF-alpha, and MCP-1) in RBL-2H3 cells after DNP stimulation. The compounds timosaponin AIII and genkwanin in XYQFT were found to be key factors in the inhibition of COX-2 and GM-CSF gene expression in mast cells. Conclusion: By regulating mast cells, XYQFT inhibited inflammatory cell infiltration, airway hypersensitivity and specific immunity in a mouse model of asthma. In addition, XYQFT synergistically inhibited the expression of the GM-CSF and COX-2 genes in mast cells through timosaponin AIII and genkwanin.

引用

页数：9