Objective: To investigate the long-term effects of radiation exposure on health and provide empirical data for radiological research. Methods: The clinical symptoms, lens, cytogenetic, immune function and tumor incidence of 45 people exposed to ionizing radiation in the dose range of 0.02-1.36 Gy were observed. Results: Compared with those of cytogenetic examination, lens opacity and tumor detection, the detection rate of lymphocyte micronuclei in the medical follow-up over-exposure group, interventional radiation group and nuclear medicine group was greater than that in the conventional radiation group, and the detection rate was the highest in the medical follow-up over-exposure group. There was a significant difference between the medical follow-up overexposure group and the interventional radiation group, and the differences among the medical follow-up overexposure group, the interventional radiation group and the conventional radiation group were statistically significant. Conclusions: Excessive exposure has certain effects on lens function, immune function and cytogenetics, and the detection rate increases with increasing dose, reflecting a certain dose-effect relationship. Genetic damage can persist in an infected person, increasing the risk of cancer.