Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean

被引:0
|
作者
Lei Wu [1 ,2 ]
Yujie Chang [2 ]
Lanfen Wang [2 ]
Shumin Wang [2 ]
Jing Wu [2 ,3 ]
机构
[1] Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River,Ministry of Education/College of Horticulture and Landscape Architecture,Southwest University
[2] Institute of Crop Sciences,Chinese Academy of Agricultural Sciences
[3] SXAU–ICS (Shanxi Agricultural University–Institute of Crop Sciences) Joint Research Center of Minor Crops,Shanxi Agricultural
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Genetic improvement of drought resistance is one of the main breeding goals for common bean, so molecular markers must be identified to facilitate drought resistance breeding. In this study, we evaluated the proline, trehalose, raffinose, and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four. The coefficients of variation ranged from 21.21% for proline content to 78.69% for stachyose content under well-watered conditions, and from 20.11% for proline content to 50.08% for trehalose content under drought stress. According to our genome-wide association analysis, 32 quantitative trait loci were associated with drought resistance, seven of which overlapped with known loci. Four hotspot regions were identified at Pv01, Pv07 and Pv11. A set of candidate genes was identified, including genes encoding MYB, bZIP, bHLH, ERF, and protein kinases. Among these genes, Phvul.001G189400, Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP, ERF and WRKY, respectively. These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean. Significant SNPs in six candidate gene regions formed different haplotypes, and phenotypic analysis revealed significant differences among the haplotypes. These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Genome-wide Association Analysis for Drought Tolerance and Associated Traits in Common Bean
    Hoyos-Villegas, Valerio
    Song, Qijian
    Kelly, James D.
    [J]. PLANT GENOME, 2017, 10 (01):
  • [2] Genome-wide association analysis of drought adaptive traits in common bean
    Dramadri, Isaac O.
    Nkalubo, Stanley T.
    Kramer, David M.
    Kelly, James D.
    [J]. CROP SCIENCE, 2021, 61 (05) : 3232 - 3253
  • [3] Genome-Wide Association Study of Agronomic Traits in Common Bean
    Kamfwa, Kelvin
    Cichy, Karen A.
    Kelly, James D.
    [J]. PLANT GENOME, 2015, 8 (02):
  • [4] Genome-wide Association Studies revealed DArTseq loci associated with seed traits in Turkish common bean germplasm
    Nadeem, Muhammad Azhar
    Baloch, Faheem Shahzad
    [J]. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2023, 47 (04) : 479 - 496
  • [5] Genome-wide association analysis of bean fly resistance and agro-morphological traits in common bean
    Nkhata, Wilson
    Shimelis, Hussein
    Melis, Rob
    Chirwa, Rowland
    Mzengeza, Tenyson
    Mathew, Isack
    Shayanowako, Admire
    [J]. PLOS ONE, 2021, 16 (04):
  • [6] Genome-Wide Association Study (GWAS) for resistance to Sclerotinia sclerotiorum in Common Bean
    Campa, Ana
    Garcia-Fernandez, Carmen
    Jose Ferreira, Juan
    [J]. GENES, 2020, 11 (12) : 1 - 16
  • [7] Genome-Wide Association Study of Agronomic and Physiological Traits Related to Drought Tolerance in Potato
    Alvarez-Morezuelas, Alba
    Barandalla, Leire
    Ritter, Enrique
    Ruiz de Galarreta, Jose Ignacio
    [J]. PLANTS-BASEL, 2023, 12 (04):
  • [8] Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean
    de Carvalho Paulino, Jean Fausto
    de Almeida, Caleo Panhoca
    Bueno, Cesar Junior
    Song, Qijian
    Fritsche-Neto, Roberto
    Morais Carbonell, Sergio Augusto
    Chiorato, Alisson Fernando
    Benchimol-Reis, Luciana Lasry
    [J]. GENES, 2021, 12 (05)
  • [9] Genome-Wide Association Study Identifies Candidate Loci Underlying Agronomic Traits in a Middle American Diversity Panel of Common Bean
    Moghaddam, Samira Mafi
    Mamidi, Sujan
    Osorno, Juan M.
    Lee, Rian
    Brick, Mark
    Kelly, James
    Miklas, Phillip
    Urrea, Carlos
    Song, Qijian
    Cregan, Perry
    Grimwood, Jane
    Schmutz, Jeremy
    McClean, Phillip E.
    [J]. PLANT GENOME, 2016, 9 (03):
  • [10] Genome-wide association study of drought-related resistance traits in Aegilops tauschii
    Qin, Peng
    Lin, Yu
    Hu, Yaodong
    Liu, Kun
    Mao, Shuangshuang
    Li, Zhanyi
    Wang, Jirui
    Liu, Yaxi
    Wei, Yuming
    Zheng, Youliang
    [J]. GENETICS AND MOLECULAR BIOLOGY, 2016, 39 (03) : 398 - 407