Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis

被引:3
|
作者
Junfang Lin a
机构
关键词
Pleurotus nebrodensis; Protoplast-mediated transformation; Green fluorescent protein; GFP expression;
D O I
暂无
中图分类号
S646 [菌类(食用菌)];
学科分类号
090202 ;
摘要
An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a "reporter" gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species.
引用
收藏
页码:819 / 824
页数:6
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