Simultaneous Nitrogen and Phosphorus Removal and Kinetics by the Heterotrophic Nitrifying Bacterium Acinetobacter junii NP1

被引：1

作者：

Yang L. ^{[1
,2
]}

Chen N. ^{[1
,2
]}

Ren Y.-X. ^{[1
,2
]}

Cui S. ^{[1
,2
]}

Wang X.-H. ^{[1
,2
]}

Xiao Q. ^{[1
,2
]}

Guo L.-K. ^{[1
,2
]}

机构：

[1] Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an

[2] Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an

来源：

Huanjing Kexue/Environmental Science | 2019年 / 40卷 / 08期

关键词：

Acinetobacter junii NP1; Aerobic denitrification; Heterotrophic nitrification; Kinetic; Simultaneous nitrogen and phosphorus removal;

D O I：

10.13227/j.hjkx.201901208

中图分类号：

学科分类号：

摘要：

Due to the problems of traditional biological nitrogen and phosphorus removal, including long process duration and high infrastructural and operational costs, the simultaneous nitrogen and phosphorus removal capabilities, influencing factors and kinetic characteristics were systematically studied using the heterotrophic nitrifier Acinetobacter junii NP1 which possesses efficient simultaneous nitrogen and phosphorus removal ability. The results showed that strain NP1 exhibited efficient heterotrophic nitrification ability with a maximum ammonia removal rate of 99.12%. Furthermore, only small amounts of nitrification intermediates were accumulated during the reaction process. Strain NP1 also adapted well to higher ammonia nitrogen loading. In addition, strain NP1 had efficient aerobic denitrification characteristics, and could utilize nitrite and nitrate for growth and metabolism, achieving a maximum removal rate of 91.40% and 95.10%, respectively. The heterotrophic nitrification process of strain NP1 was accompanied by simultaneous phosphorus accumulation, and the appropriate ratio of nitrogen to phosphorus was beneficial for the simultaneous removal of nitrogen and phosphorus. When the ratio of nitrogen to phosphorus was 5:1, the maximum ammonia nitrogen and phosphate removal rates reached 99.21% and 88.35%, respectively. The bacterial growth process of stain NP1 matched the Logistic model (R2>0.99), and the nitrogen and phosphate degradation conformed to the Compertz model (R2>0.99). The maximum conversion rates of nitrogen and phosphate (Rm) obtained by model fitting were in the order ammonia>nitrate>nitrite, and lag time (t0) was in the order nitrate>nitrite>ammonia. According to the analysis of the degradation kinetics of the matrix and the removal rate of nitrogen and phosphorus, the optimal conditions were found to be sodium succinate, C/N=10, T=30℃, and r=160 r•min-1. © 2019, Science Press. All right reserved.

引用

页码：3713 / 3721

页数：8

共 28 条

[1] Guo Y., Wang Y.Y., Zhang Z.J., Et al., Physiological and transcriptomic insights into the cold adaptation mechanism of a novel heterotrophic nitrifying and aerobic denitrifying-like bacterium Pseudomonas indoloxydans YY-1, International Biodeterioration & Biodegradation, 134, pp. 16-24, (2018)
[2] Liu Y.X., Wang Y., Li Y., Et al., Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16, Chinese Journal of Chemical Engineering, 23, 5, pp. 827-834, (2015)
[3] Huang F., Pan L.Q., Lv N., Et al., Characterization of novel Bacillus strain N31 from mariculture water capable of halophilic heterotrophic nitrification-aerobic denitrification, Journal of Bioscience and Bioengineering, 124, 5, pp. 564-571, (2017)
[4] Wang X.J., Wang W.Q., Li J., Et al., Isolation and identification of a heterotrophic nitrifier, Acinetobacter sp., and its Characteristics of Nitrogen removal, China Environmental Science, 37, 11, pp. 4241-4250, (2017)
[5] Wang X.H., Yang L., Ren Y.X., Et al., Nitrogen removal by heterotrophic nitrifying bacterium Pseudomonas putida YH and its kinetic characteristics, Environmental Science, 40, 4, pp. 1892-1899, (2019)
[6] Su J.F., Shi J.X., Huang T.L., Et al., Kinetic analysis of simultaneous denitrification and biomineralization of novel Acinetobacter sp. CN86, Marine Pollution Bulletin, 109, 1, pp. 87-94, (2016)
[7] Li C.N., Yang J.S., Wang X., Et al., Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24, Bioresource Technology, 182, pp. 18-25, (2015)
[8] Rout P.R., Bhunia P., Dash R.R., Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal, Bioresource Technology, 244, pp. 484-495, (2017)
[9] Yang L., Ren Y.X., Zhao S.Q., Et al., Isolation and characterization of three heterotrophic nitrifying-aerobic denitrifying bacteria from a sequencing batch reactor, Annals of Microbiology, 66, 2, pp. 737-747, (2016)
[10] Wang G.L., Li X.F., Zhang H., Et al., Optimization of CTN-4 to chlorothalonil-degrading conditions and a kinetics model, China Environmental Science, 33, 11, pp. 1999-2005, (2013)

← 1 2 3 →