Enhanced bioethanol production from sugarcane bagasse: combination of liquid hot water and deep eutectic solvent pretreatment for optimized enzymatic saccharification

In the present study, a sustainable pretreatment methodology combining liquid hot water and deep eutectic solvent is proposed for the efficient fractionation of hemicellulose, cellulose, and lignin from sugarcane bagasse, thereby facilitating the comprehensive utilization of both C5 and C6 sugars. The application of this combined pretreatment strategy to sugarcane bagasse led to notable enhancements in enzymatic saccharification and subsequent fermentation. Experiment results demonstrate that liquid hot water-deep eutectic solvent pretreatment yielded 85.05 +/- 0.66 g<middle dot>L-1 of total fermentable sugar (glucose: 60.96 +/- 0.21 g<middle dot>L-1, xylose: 24.09 +/- 0.87 g<middle dot>L-1) through enzymatic saccharification of sugarcane bagasse. Furthermore, fermentation of the pretreated sugarcane bagasse hydrolysate yielded 34.33 +/- 3.15 g<middle dot>L-1 of bioethanol. These findings confirm the effectiveness of liquid hot water-deep eutectic solvent pretreatment in separating lignocellulosic components, thus presenting a sustainable and promising pretreatment method for maximizing the valuable utilization of biomass resources.