High enhancement of sensitivity and reproducibility in label-free SARS-CoV-2 detection with graphene field-effect transistor sensors through precise surface biofunctionalization control

被引：0

作者：

Lozano-Chamizo, Laura ^{[1
,2
,3
]}

Márquez, Carlos ^{[4
,5
]}

Marciello, Marzia ^{[1
,2
]}

Galdon, José Carlos ^{[4
,5
]}

de la Fuente-Zapico, Elsa ^{[4
,5
]}

Martinez-Mazón, Paula ^{[4
,5
]}

Gonzalez-Rumayor, Víctor ^{[3
]}

Filice, Marco ^{[1
,2
]}

Gamiz, Francisco ^{[4
,5
]}

机构：

[1] Nanobiotechnology for Life Sciences Laboratory, Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Universidad Complutense de Madrid (UCM), Plaza Ramón y Cajal s/n, Madrid,E-28040, Spain

[2] Microscopy and Dynamic Imaging Unit, Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Calle Melchor Fernández Almagro 3, Madrid,E-28029, Spain

[3] Atrys Health, Madrid,E-28001, Spain

[4] Nanoelectronics Research Group, Department of Electronics, CITIC-UGR (Research Center for Information and Communication Technologies), University of Granada, Spain

[5] Instituto de Investigación Biosanitaria de Granada ibs.GRANADA, Granada,18012, Spain

来源：

Biosensors and Bioelectronics | 2024年 / 250卷

关键词：

The COVID-19 pandemic has taught us valuable lessons; especially the urgent need for a widespread; rapid and sensitive diagnostic tool. To this; the integration of bidimensional nanomaterials; particularly graphene; into point-of-care biomedical devices is a groundbreaking strategy able to potentially revolutionize the diagnostic landscape. Despite advancements in the fabrication of these biosensors; the relationship between their surface biofunctionalization and sensing performance remains unclear. Here; we demonstrate that the combination of careful sensor fabrication and its precise surface biofunctionalization is crucial for exalting the sensing performances of 2D biosensors. Specifically; we have biofunctionalized Graphene Field-Effect Transistor (GFET) sensors surface through different biochemical reactions to promote either random/heterogeneous or oriented/homogeneous immobilization of the Anti-SARS-CoV-2 spike protein antibody. Each strategy was thoroughly characterized by in-silico simulations; physicochemical and biochemical techniques and electrical characterization. Subsequently; both biosensors were tested in the label-free direct titration of SARS-CoV-2 virus in simulated clinical samples; avoiding sample preprocessing and within short timeframes. Remarkably; the oriented GFET biosensor exhibited significantly enhanced reproducibility and responsiveness; surpassing the detection sensitivity of conventional non-oriented GFET by more than twofold. This breakthrough not only involves direct implications for COVID-19 surveillance and next pandemic preparedness but also clarify an unexplored mechanistic dimension of biosensor research utilizing 2D-nanomaterials. © 2024 The Authors;

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[1] High enhancement of sensitivity and reproducibility in label-free SARS-CoV-2 detection with graphene field-effect transistor sensors through precise surface biofunctionalization control
Lozano-Chamizo, Laura
Marquez, Carlos
Marciello, Marzia
Galdon, Jose Carlos
de la Fuente-Zapico, Elsa
Martinez-Mazon, Paula
Gonzalez-Rumayor, Victor
Filice, Marco
Gamiz, Francisco
[J]. BIOSENSORS & BIOELECTRONICS, 2024, 250
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