Effect of Heat Treatment on the Functional Properties and Thermal Stability of Liquid Egg Yolk Hydrolyzed with Trypsin

被引:0
|
作者
Li H. [1 ]
Chen Y. [1 ]
Zhang J. [1 ]
Tang H. [1 ]
Liu X. [2 ]
Lu S. [3 ]
Chen L. [1 ]
机构
[1] Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou
[2] Beijing Deqingyuan Food Co., Ltd., Beijing
[3] Hangzhou Shunfengxiang Food Co., Ltd., Hangzhou
关键词
Egg yolk hydrolyzed with trypsin; Fourier transform infrared spectroscopy; Functional egg yolk; Heat treatment; SDS-PAGE;
D O I
10.16429/j.1009-7848.2020.10.013
中图分类号
学科分类号
摘要
Trypsin hydrolysis is an effective method to produce functional liquid egg yolk. Pasteurization is necessary for microbiological safety in liquid egg production. This study investigated the effects of different heat treatments (60, 64, 68℃ and 72℃, 4 min) on the functional properties and thermal stability of liquid egg yolk hydrolyzed with trypsin. The results showed that functional properties of liquid egg yolk hydrolyzed with trypsin, such as emulsifying activities and emulsion stability, protein solubility, the water absorption capacity and the oil absorption capacity, foaming capacity and foam stability, were significantly higher (P < 0.05) than that of liquid egg yolk without trypsin hydrolysis. Emulsifying activities, protein solubility and foaming capacity of liquid egg yolk with/without trypsin hydrolysis showed significantly decreasing with the temperature increasing. The result of SDS-PAGE illustrated that trypsin hydrolysis could obviously change the distribution of protein molecular weight in liquid egg yolk, and higher temperature heat treatment (68℃ and 72℃) resulted in significant protein aggregation. Fourier transform infrared spectroscopy results confirmed that trypsin hydrolysis and heat treatment had a significantly effect on egg yolk -OH functional groups. © 2020, Editorial Office of Journal of CIFST. All right reserved.
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页码:105 / 114
页数:9
相关论文
共 32 条
  • [1] 1
  • [2] (2018)
  • [3] 7, pp. 44-47
  • [4] BUXMANN W, BINDRICH U, STRIJOWSKI U, Et al., Influencing emulsifying properties of egg yolk by enzymatic modification with phospholipase D. Part 2: structural changes of egg yolk due to incubation, Colloids Surf B Biointerfaces, 76, 1, pp. 192-198, (2010)
  • [5] 8
  • [6] DAIMER K, KULOZIK U., Oil-in-water emulsion properties of egg yolk: Effect of enzymatic modification by phospholipase A2, Food Hydrocolloids, 23, 5, pp. 1366-1373, (2008)
  • [7] JIN Y, HUANG D, DING T, Et al., Effect of phospholipase A1 on the physicochemical and functional properties of hen's egg yolk, plasma and granules, Journal of Food Biochemistry, 37, 1, pp. 70-79, (2013)
  • [8] DAIMER K, KULOZIK U., Impact of a treatment with phospholipase A2 on the physicochemical properties of hen egg yolk, Journal of Agricultural and Food Chemistry, 56, 11, pp. 4172-4180, (2008)
  • [9] KIM H J, DECKER E A, MCCLEMENTS D J., Influence of protein concentration and order of addition on thermal stability of beta-lactoglobulin stabilized n-hexadecane oil-in-water emulsions at neutral pH, Langmuir the ACS Journal of Surfaces and Colloids, 21, 1, pp. 134-139, (2005)
  • [10] (2010)