Afforestation enhances glomalin-related soil protein content but decreases its contribution to soil organic carbon in a subtropical karst area

Afforestation on degraded croplands has been proposed as an effective measure to promote ecosystem functions including soil organic carbon (SOC) sequestration. Glomalin-related soil protein (GRSP) plays a crucial role in promoting the accumulation and stability of SOC. Nevertheless, mechanisms underlying the effects of afforestation on GRSP accumulation have not been well elucidated. In the present study, 14 pairs of maize fields and plantation forests were selected using a paired-site approach in a karst region of southwest China. By measuring soil GRSP and a variety of soil biotic and abiotic variables, the pattern of and controls on GRSP accumulation in response to afforestation were explored. The average content of total GRSP (T-GRSP) and its contribution to SOC in the maize field were 5.22 +/- 0.29 mg g-1 and 42.33 +/- 2.25%, and those in the plantation forest were 6.59 +/- 0.32 mg g-1 and 25.77 +/- 1.17%, respectively. T-GRSP content was increased by 26.4% on average, but its contribution to SOC was decreased by 39.1% following afforestation. T-GRSP content decreased as soil depth increased regardless of afforestation or not. Afforestation increased T-GRSP indirectly via its positive effects on arbuscular mycorrhizal fungi biomass, which was stimulated by afforestation through elevating fine root biomass or increasing the availability of labile C and N. The suppressed contribution of T-GRSP to SOC following afforestation was due to the relatively higher increase in other SOC components than T-GRSP and the significant increase of soil C:N ratio. Our study reveals the mechanisms underlying the effects of afforestation on T-GRSP accumulation, and is conducive to improving the mechanistic understanding of microbial control on SOC sequestration following afforestation.