Determination of PCDD/Fs and Dioxin-like PCBs in Fish Based on GC-MS/MS

被引：0

作者：

Lu J. ^{[1
]}

Shen T. ^{[1
]}

Jiao Y. ^{[1
]}

Cui F. ^{[2
]}

Zhu S. ^{[1
]}

Cheng J. ^{[1
]}

Yi X. ^{[1
]}

Fu S. ^{[1
]}

机构：

[1] Technology Center of Changsha Customs, Hunan Key Lalioratory of Food Safety Science & Technology, Changsha

[2] Science and Technology Research Center of China Customs, Beijing

来源：

Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 09期

关键词：

dioxin-like PCBs; fish; GC-MS/MS; PCDD/Fs;

D O I：

10.16429/J.1009-7848.2023.09.040

中图分类号：

学科分类号：

摘要：

Based on GC-MS/MS, a stable isotope dilution method was established for the determination of dioxins and dioxin like polychlorinated biphenyls in fish. The sample was extraeted by rapid solvent extraetor for 3 times, and the fat was removed by acidified silica gel. Then, after purification by multi—layer silica gel column, alkaline alumina column and activated carbon column, the elution samples containing PCDD/Fs and DL-PCBs were collected respectively. Finally, it is concentrated and redissolved for GC-MS/MS determination. In this experiment, the extraction, purification and determination steps of samples were optimized and discussed. The results show that in the purification step, PCDD/Fs and DL—PCBs are greatly affected by the volume of washing liquid and collection step. PCDD/Fs and DL—PCBs are collected separately. Firstly, the collection solution of PCDD/Fs was measured, and then it was combined with the collection solution of DL-PCBs to complete the determination of DL-PCBs, thereby accurately quantifying DL-PCBs. When the fish was detected, the internal standard recoveries of PCDD/Fs and DL-PCBs were 63.3%-106.8% and RSD was 0.4%-9.6%; The recovery rate of the target substance is 93.6%—114.5%, and the RSD is 0.9%—9.2%, which meets the relevant requirements of the national standard of GB 5009.205. It is suitable for the determination of PCDD/Fs and DL-PCBs in actual fish samples. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.

引用

页码：376 / 387

页数：11

共 32 条

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