Animal Manure Fertilization Promotes Antibiotic Resistance Gene Dissemination Among Manure, Soil, and Vegetables

被引：5
|
作者：
Zhang H.-N. [1 ]
Dong M.-J. [1 ]
Zhou Y.-F. [2 ]
Sun J.-X. [1 ]
Chang M.-J. [1 ]
Zhai Z.-Z. [3 ]
机构：
[1] College of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang
[2] Animal Husbandry Bureau of Daiyue Region, Tai'an
[3] Center Hospital of Tai'an City, Tai'an
来源：
Huanjing Kexue/Environmental Science | 2021年 / 42卷 / 04期
关键词：
Antibiotic resistant genes (ARG); Bacterial community; Cichorium endivia L; Endophyte; High-throughput quantitative PCR (HT-qPCR); Poultry manure fertilization;
D O I：
10.13227/j.hjkx.202009038
中图分类号：
学科分类号：
摘要：
Increasing antibiotic resistant genes (ARG) are being detected in animal manure-amended soil, causing public concerns. However, the effects of animal manure fertilization on the ARG of vegetable endophytes remain unknown. In this study, high-throughput quantitative PCR (HT-qPCR) was used to explore the effects of poultry manure fertilization on the bacterial community and resistome in the soil, root endophytes, and leaf endophytes from Cichorium endivia L.. The results indicated that poultry manure fertilization not only enhanced the number of ARG in soil and root endophytes, but also increased the abundance of ARG in the soil, root endophytes, and leaf endophytes. The relationship analysis demonstrated that the ARG profile in the soil and vegetable endophytes was significantly correlated with the bacterial community, and the ARG abundance was highly correlated with Proteobacteria, Acidobacteria, Actinobacteria, and Cyanobacteria. In addition, the overlapping ARG between the Cichorium endivia L. endophyte and soil indicated the internal spread route of the ARG from the manure to Cichorium endivia L through the soil. In conclusion, poultry manure fertilization increased the diversity and abundance of the ARG in vegetables via the manure-soil-vegetable pathway. © 2021, Science Press. All right reserved.
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页码：2080 / 2088
页数：8
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