Molecular characteristics and antimicrobial resistance profiles of Staphylococcus aureus isolates from burns

被引:0
|
作者
Kadhum, Duaa Adnan [1 ]
Hamad, Entisar Mahdi [1 ]
Fahad, Morooj Ali [2 ]
机构
[1] Middle Tech Univ, Baqubah Tech Inst, Dept Med Labs Tech, Baghdad, Iraq
[2] Middle Tech Univ, Tech Inst, Community Hlth Dept, Baghdad, Iraq
关键词
Staphylococcus aureus; Antibiotic susceptibility test (AST); arcC; aroE; Glp genes;
D O I
10.1007/s11033-024-09837-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background Burns are a problem that affects millions of individuals around the world. Objective This research aimed to analyze the genetic characteristics and antibiotic resistance of Staphylococcus aureus strains isolated from patients with burns. Identifying the genetic variations of three local strains of Staphylococcus aureus isolated from burns. Materials and methodsSwab samples were collected from eighty sources (burns). Using sterile swabs containing media collected from patients treated at Baqubah Teaching Hospital between July 2022 and the end of September 2022, these samples were then cultured on blood agar and brain heart infusion agar. A total of twenty-four hours were spent incubating the cultured samples in an aerobic environment at 37 degrees C. During this time, isolated growing colonies showed characteristic growth, color, and hemolysis, while suspicious colonies were cultured for further identification. Results Our results indicated the presence of several polymorphisms that were distributed in the investigated samples. However, almost all observed variations were concentrated only in the S2 isolates. The construction of phylogenetic trees confirmed this notion by positioning these S2-based amplicons to distinct categories within Staph. aureus organisms. Furthermore, the phylogenetic tree offered additional tools for the guaranteed identity of the samples that were analyzed. Consequently, the utilization of the PCR-sequencing approach in three DNA samples belonging to these local bacterial isolates has resulted in the confirmation of the identity of this strain. However, particular emphasis should be placed on S2 isolate as it has special variants that differ from its mates, in terms of its metabolic as well as phylogenetic consequences. Therefore, S2 isolates may represent a new strain that requires a whole genome sequencing strategy to validate its identity within Staph. aureus organisms. S.aureus resistance was 100% (Augmentin and Tetracycline), and 90% (Azithromycin and Trimethoprim), while Cefotaxime and Chloramphenicol recorded (75%, and 85%) respectively.
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页数:11
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