Preparation of leuco malachite green imprinting sensor based on multiple technologies and its application

被引：0

作者：

Qin L. ^{[1
]}

Li D. ^{[1
]}

Tian J. ^{[1
]}

Han J. ^{[1
]}

Zhang Y. ^{[2
]}

Li J. ^{[2
]}

Gao W. ^{[1
]}

机构：

[1] College of Food and Biology, Hebei University of Science and Technology, Hebei, Shijiazhuang

[2] Shijiazhuang Food and Drug Inspection Center, Hebei, Shijiazhuang

来源：

Huagong Jinzhan/Chemical Industry and Engineering Progress | 2022年 / 41卷 / 11期

关键词：

computer molecular simulation technology; electrochemical analysis technology; leuco malachite green; molecularly imprinted sensor; ultraviolet spectroscopy;

D O I：

10.16085/j.issn.1000-6613.2022-0151

中图分类号：

学科分类号：

摘要：

In view of the harm of leuco malachite green (LMG) to human health, it is important to establish a sensitive and rapid method for the detection of LMG residues in aquatic products. In this study, a novel molecularly imprinted electrochemical sensor (MIECS) for LMG detection was successfully prepared by the fusion of variety technologies, such as computer simulation technology, ultraviolet spectroscopy, self-assembled technique and electrochemical analysis technology. In the experiment, based on nanomaterial modified electrodes, LMG was used as the template molecule, and 4-aminophenol (4-ATP) was selected as the best functional monomer by the use of ultraviolet spectroscopy combined with computer simulation. The configuration, action forms and binding energies of the LMG and 4-ATP preassembly systems were simulated and calculated by using Gaussian 09. The morphology and chemical characterization of the membrane were characterized by scanning electron microscope and infrared spectrometer, and the imprinted effect and selection performance of the sensor were investigated by cyclic voltammetry and square-wave voltammetry. The sensor was applied for the rapid detection of food safety. The results showed that the template molecule and functional monomer mainly formed the LMG-2(4ATP) complex through conjugation action. The sensor demonstrated good performance and could specifically recognize the LMG and its structural analogs. The linear response range of this method was 3.3×10−11—1.0×10−6mol/L with a good linear relationship, the detection limit was 1.0×10−11mol/L, the spiked sample recoveries were between 85.5% and 101.2% and the relative standard deviations were 1.28%—2.48%. It was suitable for the sensitive and rapid detection of LMG residues in aquatic products. In the future, MIECS would be more sensitive, accurate, fast and miniaturized, and applied in more fields. © 2022 Chemical Industry Press. All rights reserved.

引用

页码：6018 / 6028

页数：10

共 26 条

[1] ARSLAN Taner, GUNEY Sevgi, GUNEY Orhan, Electrochemical and spectrophotometric detection of malachite green in aqueous system using imprinted sol-gel polymer-capped quantum dots, International Journal of Environmental Analytical Chemistry, 100, 7, pp. 808-824, (2020)
[2] WANG Meiyin, ZHANG Xinying, LIN Linlin, Et al., Treatment of malachite green by immobilized laccase in ZrO2 via biomineralization, Chemical Industry and Engineering Progress, 37, 7, pp. 2798-2805, (2018)
[3] ZHAO Dan, Simultaneous determination of malachite green, crystal violet and their metabolite residues in cultured fish by high performance liquid chromatography-tandem mass spectrometry, China Food Safety Magazine, 1, pp. 90-93, (2022)
[4] SRIVASTAV A K, ROY D., Acute toxicity of malachite green (Triarylmethane dye) and pyceze (Bronopol) on carbohydrate metabolism in the freshwater fish Heteropneustes fossilis (Bloch.) [J], International Journal of Fisheries and Aquatic Studies, 6, pp. 27-30, (2018)
[5] ZHANG Leilei, ZHANG Yiying, TANG Yurong, Et al., Magnetic solid-phase extraction based on Fe3O4/graphene oxide nanoparticles for the determination of malachite green and crystal violet in environmental water samples by HPLC, International Journal of Environmental Analytical Chemistry, 98, 3, pp. 215-228, (2018)
[6] WANG Yulei, LIAO Keren, HUANG Xiaojia, Et al., Simultaneous determination of malachite green, crystal violet and their leuco-metabolites in aquaculture water samples using monolithic fiber-based solid-phase microextraction coupled with high performance liquid chromatography, Analytical Methods, 7, 19, pp. 8138-8145, (2015)
[7] GAO Na, WANG Wenlan, GUO Jun, Et al., Uncertainty evaluation for determination of malachite green in Gadus by high performance liquid chromatography, Journal of Food Safety & Quality, 10, 3, pp. 653-657, (2019)
[8] YU Zhou, GU Xiaofeng, DING Baiyu, Determination of malachite green in aquatic products and their processed products by liquid chromatography-mass spectrometry, Farm Products Processing, 2, pp. 53-56, (2020)
[9] YAMASHITA Takeshi, NISHIKAWA Kiyofumi, SHINOZAKI Fumiyoshi, Et al., Validation study of analysis of malachite green in broiled eels (kabayaki) by LC-MS/MS, Food Hygiene and Safety Science, 56, 1, pp. 31-36, (2015)
[10] ASCARI Jociani, DRACZ Sergio, SANTOS Flavio A, Et al., Validation of an LC-MS/MS method for malachite green (MG), leucomalachite green (LMG), crystal violet (CV) and leucocrystal violet (LCV) residues in fish and shrimp, Food Additives & Contaminants: Part A, 29, 4, pp. 602-608, (2012)

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