Chemical modifications, ions and water molecules in the sub-2 Å resolution structure of the human 80S ribosome

被引：0
|
作者：
Klaholz, Bruno P. [1 ]
机构：
[1] Inst Genet & Mol & Cellular Biol, Ctr Integrat Biol CBI, Illkirch Graffenstaden, France
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2024年 / 31卷 / 08期
关键词：
D O I：
10.1038/s41594-024-01275-w
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using next-generation cryo-EM and mass spectrometry, we identified 235 chemical modifications in the sub-2 & Aring; resolution structure of the full human 80S ribosome. The newly identified rRNA modifications were found to create new hydrogen bond patterns for riboses and uridines. Ion visualization revealed that Mg2+-associated water molecules are variably substituted by side chains. This study provides the molecular basis for the stabilization of A-U or A-Psi base pairs and RNA-protein interactions.
引用
收藏
页码：1152 / 1153
页数：2
相关论文
共 17 条
  • [1] Visualization of chemical modifications in the human 80S ribosome structure
    S. Kundhavai Natchiar
    Alexander G. Myasnikov
    Hanna Kratzat
    Isabelle Hazemann
    Bruno P. Klaholz
    [J]. Nature, 2017, 551 : 472 - 477
  • [2] Visualization of chemical modifications in the human 80S ribosome structure
    Natchiar, S. Kundhavai
    Myasnikov, Alexander G.
    Kratzat, Hanna
    Hazemann, Isabelle
    Klaholz, Bruno P.
    [J]. NATURE, 2017, 551 (7681) : 472 - +
  • [3] The structure of the human 80S ribosome at 1.9 Å resolution reveals the molecular role of chemical modifications and ions in RNA
    Holvec, Samuel
    Barchet, Charles
    Lechner, Antony
    Frechin, Leo
    De Silva, S. Nimali T.
    Hazemann, Isabelle
    Wolff, Philippe
    von Loeffelholz, Ottilie
    Klaholz, Bruno P.
    [J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2024, 31 (08) : 1251 - 1264
  • [4] Landscape of the complete RNA chemical modifications in the human 80S ribosome
    Taoka, Masato
    Nobe, Yuko
    Yamaki, Yuka
    Sato, Ko
    Ishikawa, Hideaki
    Izumikawa, Keiichi
    Yamauchi, Yoshio
    Hirota, Kouji
    Nakayama, Hiroshi
    Takahashi, Nobuhiro
    Isobe, Toshiaki
    [J]. NUCLEIC ACIDS RESEARCH, 2018, 46 (18) : 9289 - 9298
  • [5] Structure of the human 80S ribosome
    Heena Khatter
    Alexander G. Myasnikov
    S. Kundhavai Natchiar
    Bruno P. Klaholz
    [J]. Nature, 2015, 520 : 640 - 645
  • [6] Structure of the human 80S ribosome
    Natchiar, S. Kundhavai
    Khatter, Heena
    Myasnikov, Alexander G.
    Klaholz, Bruno P.
    [J]. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2015, 71 : S33 - S33
  • [7] Structure of the human 80S ribosome
    Khatter, Heena
    Myasnikov, Alexander G.
    Natchiar, S. Kundhavai
    Klaholz, Bruno P.
    [J]. NATURE, 2015, 520 (7549) : 640 - U338
  • [8] Structure of the mammalian 80S ribosome at 8.7 Å resolution
    Chandramouli, Preethi
    Topf, Maya
    Menetret, Jean-Francois
    Eswar, Narayanan
    Cannone, Jamie J.
    Gutell, Robin R.
    Sali, Andrej
    Akey, Christopher W.
    [J]. STRUCTURE, 2008, 16 (04) : 535 - 548
  • [9] High-Resolution Structure of the Eukaryotic 80S Ribosome
    Yusupova, Gulnara
    Yusupov, Marat
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83, 2014, 83 : 467 - 486
  • [10] Structure of the actively translating plant 80S ribosome at 2.2 Å resolution
    Julia Smirnova
    Justus Loerke
    Gunnar Kleinau
    Andrea Schmidt
    Jörg Bürger
    Etienne H. Meyer
    Thorsten Mielke
    Patrick Scheerer
    Ralph Bock
    Christian M. T. Spahn
    Reimo Zoschke
    [J]. Nature Plants, 2023, 9 : 987 - 1000
12