Identification and Cold Stress-responsive Expression Analysis of Dehydroascorbate Reductase Gene in Hami Melon(Cucumis melo var. saccharinus)

被引：0
|
作者：
Song W. [1 ]
Zhang Q. [1 ]
Ning M. [1 ]
Zhou F. [1 ]
Yang X. [1 ]
Shan C. [1 ]
Tang F. [1 ]
机构：
[1] Engineering Research Center for Storage and Processing of Xinjiang Characteristic Fruits and Vegetables, Ministry of Education, College of Food, Shihezi University, Shihezi
来源：
Shipin Kexue/Food Science | 2022年 / 43卷 / 02期
关键词：
Cold stress; Dehydroascorbate reductase; Glutathione S-transferase; Hami melon;
D O I：
10.7506/spkx1002-6630-20201109-084
中图分类号：
学科分类号：
摘要：
To clarify the basic properties of the dehydroascorbate reductase (DHAR) gene in Hami melon and its expression level during cold stress, we investigated the basic information, multiple sequence alignment, phylogenetic relationship, collinearity and domain characteristics of two DHAR genes, protein three-dimensional (3D) models and protein-protein interaction, as well as ascorbic acid (AsA) contents, DHAR activities and DHAR gene expression levels under cold stress, and we performed gene set enrichment analysis (GSEA). The results showed that the lengths of the proteins encoded by the two CmDHAR genes consisted of 297 and 206 amino acids, respectively. Their isoelectric points were 8.67 and 5.99, respectively. Their molecular masses were 33 082.9 and 22 891.6 Da, respectively. Multiple sequence alignment analysis showed that the CmDHAR genes had a highly conserved glutathione S-transferase (GST) N-terminal functional domain.Gene structure analysis indicated that CmDHAR contained six exons, six conserved motifs and three types of cis-acting elements. Collinearity analysis indicated that Hami melon was evolutionarily closer to zucchini, winter squash and pumpkin. The 3D models revealed that the secondary structure of CmDHAR2 mainly included α-helix, random coil, extended strand and β-turn. Protein-protein interaction analysis showed that CmDHAR family members were closely related to other GST family members. The AsA content, DHAR activity and CmDHAR gene expression level in the cold-tolerant cultivar"Jiashi-310" (JS) were significantly higher than those in the cold-sensitive cultivar "Golden Empress-308" (GE) under cold stress. GSEA results revealed that the CmDHAR genes participated in the biological processes of 'structural molecule activity', 'macromolecular complex', 'membrane-enclosed lumen' and 'antioxidant activity'. This study has proved that the CmDHAR genes play an important role in the resistance of Hami melon to cold stress. © 2022, China Food Publishing Company. All right reserved.
引用
收藏
页码：117 / 125
页数：8
相关论文
共 36 条
  • [1] SONG W, TANG F X, CAI W C, Et al., iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus)after cold storage, BMC Genomics, 21, 1, (2020)
  • [2] NING M, TANG F X, ZHANG Q, Et al., The quality of Gold Queen Hami melons stored under different temperatures, Scientia Horticulturae, 243, pp. 140-147, (2019)
  • [3] SHI X F, WANG X B, CHENG F, Et al., iTRAQ-based quantitative proteomics analysis of cold stress-induced mechanisms in grafted watermelon seedlings, Journal of Proteomics, 192, pp. 311-320, (2019)
  • [4] PIERO A R L, PUGLISI I, RAPISARDA P, Et al., Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage, Journal of Agricultural and Food Chemistry, 53, 23, pp. 9083-9088, (2005)
  • [5] BRENTNER L B, MUKHERJI S T, MERCHIE K M, Et al., Expression of glutathione S-transferases in poplar trees (Populus trichocarpa)exposed to 2, 4, 6-trinitrotoluene (TNT)[J], Chemosphere, 73, 5, pp. 657-662, (2008)
  • [6] WANG M Q, WANG Y F, ZHANG Y F, Et al., Comparative transcriptome analysis of salt-sensitive and salt-tolerant maize reveals potential mechanisms to enhance salt resistance, Genes &Genomics, 41, 7, pp. 781-801, (2019)
  • [7] XU J, TIAN Y S, XING X J, Et al., Over-expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis, Physiologia Plantarum, 156, 2, pp. 164-175, (2016)
  • [8] 41, 7, (2020)
  • [9] LIU Y J, HAN X M, REN L L, Et al., Functional divergence of the glutathione S-transferase supergene family in physcomitrella patens reveals complex patterns of large gene family evolution in land plants, Plant Physiology, 161, 2, pp. 773-786, (2013)
  • [10] JIANG Y C, HUANG C Y, WEN L, Et al., Dehydroascorbate reductase cDNA from sweet potato (Ipomoea batatas L. Lam): expression, enzyme properties, and kinetic studies, Journal of Agricultural and Food Chemistry, 56, 10, pp. 3623-3627, (2008)
1234