Optimization of supercritical fluid extraction of bioactive compounds from Ammodaucus leucotrichus fruits by using multivariate response surface methodology

This study explored supercritical fluid extraction (SFE) of bioactive molecules from Ammodaucus leucotrichus seeds, a little-known source of potent bioactive compounds. Response surface methodology assessed extraction parameters (pressure, temperature, and ethanol co-solvent) and their effects on global yield, total phenolic compound (TPC), total carbohydrate content (TCC), and anti-Alzheimer activity. Optimization via a BoxBehnken design identified the optimal conditions: 15 % ethanol, 300 bar, and 70 degrees C. Chemical profiling using GC-MS and UHPLC-q-tof-MS/MS detected over 41 metabolites. In addition, a biorefinery sequential extraction process was performed to obtain extracts with high neuroprotective potential. The SFE extracts exhibited modest AChE inhibition. Notably, sequential SFE and PLE extractions yielded extracts with enhanced antioxidant activity compared to separate processes. In conclusion, Ammodaucus leucotrichus presents a promising natural source of secondary metabolites. Its potential health benefits warrant further exploration, emphasizing the need for continued research.