Rapid Screening of 28 Kinds of Colorant in Meat Product by High Resolution Mass Spectrometry

被引：0

作者：

Zhang H. ^{[1
]}

Liu Z. ^{[2
]}

Li Z. ^{[3
]}

Wang J. ^{[4
]}

机构：

[1] Weihai Ocean Vocational College, Weihai, 264300, Shandong

[2] Huangdao Customs, Qingdao, 266555, Shandong

[3] Qingdao Agricultural University, Qingdao, 266109, Shandong

[4] Weihai Customs Technical Center, Weihai, 264205, Shandong

来源：

Journal of Chinese Institute of Food Science and Technology | 2020年 / 20卷 / 07期

关键词：

High resolution mass spectrometry; Meat product; Rapid screening; Synthetic colorant; Ultra performance liquid chromatography;

D O I：

10.16429/j.1009-7848.2020.07.028

中图分类号：

学科分类号：

摘要：

Objective: To develop a rapid and simple method for the quantification of 28 kinds of synthetic colorant in meat product by ultra performance liquid chromatography quadrupole electrostatic field obitrap high resolution mass spectrometry. Methods: The samples were extracted by acetonitrile and ethyl acetate and cleaned up. The column was used as chromatography column to separate the synthetic colorant with a gradient elution of the mobile phase. The 28 kinds of synthetic colorant were analyzed in positive and negative mode. The retention time, accurate mass of parent ion and relative isotope abundance were used, while accurate mass of fragment ions was used for confirmation. Results: The linearities were good (R2 > 0.99). The limits of detection (LODs) were between 2.5 and 500 μg/kg. The recoveries were in the range of 73.5%-109.2% and the relative standard deviations (RSDs) were 4.9%-10.0%. Conclusion: The method is simple, rapid and reliable, which is suitable for the rapid qualitative screening and quantitative analysis of 28 kinds of synthetic colorant in meat product. © 2020, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：229 / 236

页数：7