Pressure-resistant and portable array gas membrane device for rapid Escherichia coli detection in infant milk powder via smartphone colorimetry with all-in-one preparation strategy

被引：0

作者：

Yu, Laishui ^{[1
]}

Huang, Liye ^{[1
]}

Wang, Zhuangyuan ^{[1
]}

Xiong, Yuhao ^{[3
]}

Li, Gongke ^{[2
]}

Chen, Zhengyi ^{[1
]}

机构：

[1] Guilin Med Univ, Guangxi Engn Res Ctr Pharmaceut Mol Screening & Dr, Sch Pharm, Guangxi Key Lab Drug Discovery & Optimizat, Guilin 541199, Peoples R China

[2] Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Peoples R China

[3] Hezhou Univ, Sch Food & Biol Engn, Guangxi Key Lab Hlth Care Food Sci & Technol, Hezhou 542899, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2024年 / 412卷

基金：

中国国家自然科学基金;

关键词：

Pressure-resistant and portable array gas; membrane; Separation; Rapid detection; Escherichia coli(E.coli); Infant milk powder; Smartphone colorimetric sensing;

D O I：

10.1016/j.snb.2024.135791

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study presents a novel Pressure-Resistant and Portable Array Gas Membrane Separation (PAGMS) device for the rapid, smartphone-based detection of Escherichia coli ( E. coli ) in infant milk powder. The PAGMS device efficiently separates acetic acid, a byproduct of E. coli growth, enhancing detection sensitivity. Utilizing bromocresol violet as an indicator, this method quantifies E. coli based on colorimetric changes, with UV spectrum absorbance shifts indicating a linear correlation (R 2 = 0.9864) within 5.0 -120 mu mol/L acetic acid concentration. Visual MINTEQ simulations validate the mechanism behind the indicator 's response. The approach surpasses traditional plate culture methods in terms of sample preparation time, achieving detection within a comparable timeframe of 15 min, and can concurrently process up to 48 samples, thereby substantially enhancing analytical throughput while detecting E. coli with a limit of detection of 5 cfu/g. This method demonstrates enhanced sensitivity, speed, and high-throughput capabilities, offering significant advantages in food safety monitoring.

引用

页数：11