Radiocomplexation, Quality Control and Bioevaluation of [99mTc]tricarbonyl Rolipram for Brain Imaging in Mice

In this work, the complex of [Tc-99m]tricarbonyl rolipram was labeled utilizing a [Tc-99m]tricarbonyl core. Many optimal parameters have been used such as substrate (100 mu g), pH of the reaction mixture (pH 9), reaction time (30 min), as well as temperature (100 degrees C), providing an optimal radiochemical purity of 97.0%. Biodistribution investigations of our radiotracer, [Tc-99m]tricarbonyl rolipram, were conducted on normal Swiss Albino mice. The results indicated maximum brain uptakes of 8.2 %ID/g tissue at 10 min post injection (p.i.), which cleared from the brain with time until it reached 1.90 +/- 0.17% at 1 h p.i. Therefore, [Tc-99m]tricarbonyl rolipram complex may be an excessively bio-selective receptor-tracer for brain ulcer imaging through the phosphodiesterase-4 inhibitor.