Optimization of Extraction Conditions and Characterization of Lipases from Pequi (Caryocar Brasiliense Camb.) Fruit By-Product

被引：0

作者：

dos Santos, Karine Amaral ^{[1
]}

Muniz, Izabella de Carvalho Batista ^{[1
]}

Nascimento, Priscilla Amaral ^{[1
]}

Borges, Jessica Ferreira ^{[1
]}

Alves, Annie Nolasco ^{[1
]}

Jardim, Matheus Firmino ^{[1
]}

Lima, Thais Santos Moraes ^{[1
]}

Bonomo, Renata Cristina Ferreira ^{[1
]}

机构：

[1] State Univ Southwest Bahia, Proc Engn Lab, BR-45700000 Itapetinga, Ba, Brazil

来源：

WASTE AND BIOMASS VALORIZATION | 2024年 / 15卷 / 09期

关键词：

Downstream Process; Experimental Design; Plant Enzyme; Solid-liquid Extraction; Thermostability; PROTEIN; ENZYME;

D O I：

10.1007/s12649-024-02539-8

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study evaluated the optimization of solid-liquid extraction of lipase from pequi shell using response surface methodology (RSM) as a statistical tool to determine the best conditions (time and extraction solution pH) for obtaining an extract with higher lipolytic activity (U/mg). A significant effect of extraction time and pH of the medium on the response variable was observed, with a maximum activity of approximate to 189.55 U/mg under the optimized conditions. The extract was characterized for maximum efficiency of hydrolysis reaction (EHRmax) and stability as a function of pH (4-10) and temperature (30-80 degrees C). EHRmax (3911.18 +/- 28.28 U/mg) was observed at pH 5 and 60 degrees C, and the enzyme stability (relative activity >= 60%) was observed in the pH 5-7 range up to 180 min. Therefore, the experimental design used for the extraction was efficient, and the model found by the response surface represented the empirical values.

引用

页码：5527 / 5537

页数：11

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