Defect-free SLM-IN 738LC superalloy samples were fabricated using selective laser melting (SLM). The as-built samples exhibit a cellular structure with fine carbides, gamma ' phases, and high-density dislocations at the cell boundary and nano-size gamma ' phases in the interior, resulting in excellent deformation resistance and favorable mechanical properties at room temperature, with a yield strength (YS) of 970 MPa, an ultimate tensile strength (UTS) of 1263 MPa, and an elongation (EL) of 33%. This study investigates the evolution behavior of the gamma ' phase at different solid solution treatment (SHT) temperatures and its impact on alloy strength and toughness. The 1020 + AHT sample exhibits a uniform distribution of coarse gamma ' phases. The samples subjected to 1070 + AHT and 1120 + AHT display a bimodal distribution of gamma ' phases. When the SHT temperature was further increased, fine spherical gamma ' phases are evenly distributed in the 1160 + AHT and 1200 + AHT samples. Moreover, the gamma/gamma ' eutectics at the grain boundaries gradually dissolve with increasing SHT temperature, whereas the size and fraction of carbides increase. The room temperature tensile test of the 1070 + AHT sample demonstrated a favorable balance between strength and plasticity (YS = 1217 MPa, UTS = 1472 MPa, EL = 9.25%). This is attributed to the synergistic strengthening effect of the bimodal distribution of the gamma ' phase. The temperature-dependent deformation behavior of the 1070 + AHT sample was analyzed at 23 degrees C, 800 degrees C, 900 degrees C, and 1000 degrees C. At 23 degrees C, numerous dislocations accumulated around the gamma/gamma ' interfaces. When the temperature increased to 800-900 degrees C, the dislocations shearing gamma ' phases is activated and the alloy remained high strength. At 1000 degrees C, both the dislocation shearing and dislocation bypassing mechanisms coexist, resulting in a decrease in the strength of the alloy and an increase in its plasticity. This work provides scientific and theoretical support for SLM-IN 738LC parts with favorable properties.