Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

被引：0
|
作者：
Dahl, Anja Lindis [1 ,4 ]
Friis, Martin Barfred [1 ,5 ]
Hallberg, Hanne Wiese [1 ]
Kristiansen, Gitte Qvist [2 ]
Holzknecht, Barbara Juliane [1 ,3 ]
机构：
[1] Copenhagen Univ Hosp Herlev & Gentofte, Dept Clin Microbiol, Herlev, Denmark
[2] Copenhagen Univ Hosp Amager & Hvidovre, Dept Clin Microbiol, Hvidovre, Denmark
[3] Univ Copenhagen, Dept Clin Med, Copenhagen, Denmark
[4] Copenhagen Univ Hosp Rigshosp, Dept Infect Dis, CHIP, Blegdamsvej 9, Copenhagen, Denmark
[5] Symbion, QIAGEN Denmark, Fruebjergvej 3, Copenhagen, Denmark
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2024年 / 109卷 / 02期
关键词：
Vancomycin-resistant enterococci; VRE; vanB; Enrichment; Carriage; DIAGNOSTIC-ACCURACY; ASSAY;
D O I：
10.1016/j.diagmicrobio.2024.116233
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.
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页数：3
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